Bio-sample detection on paper-based devices with inkjet printer-sprayed reagents

Wun Hong Liang, Chien Hung Chu, Ruey Jen Yang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The reagent required for bio-sample detection on paper-based analytical devices is generally introduced manually using a pipette. Such an approach is time-consuming; particularly if a large number of devices are required. Automated methods provide a far more convenient solution for large-scale production, but incur a substantial cost. Accordingly, the present study proposes a low-cost method for the paper-based analytical devices in which the biochemical reagents are sprayed onto the device directly using a modified commercial inkjet printer. The feasibility of the proposed method is demonstrated by performing aspartate aminotransferase (AST) and alanine aminotransferase (ALT) tests using simple two-dimensional (2D) paper-based devices. In both cases, the reaction process is analyzed using an image-processing-based colorimetric method. The experimental results show that for AST detection within the 0-105 U/l concentration range, the optimal observation time is around four minutes, while for ALT detection in the 0-125 U/l concentration range, the optimal observation time is approximately one minute. Finally, for both samples, the detection performance of the sprayed-reagent analytical devices is insensitive to the glucose concentration.

Original languageEnglish
Pages (from-to)6-11
Number of pages6
JournalTalanta
Volume145
DOIs
Publication statusPublished - 2015 Dec 1

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Bio-sample detection on paper-based devices with inkjet printer-sprayed reagents'. Together they form a unique fingerprint.

  • Cite this