Bioactive constituents from the roots of panax japonicus var. major and development of a LC-MS/MS method for distinguishing between natural and artifactual compounds

Hsiu Hui Chan, Tsong Long Hwang, Mopur Vijaya Bhaskar Reddy, Ding Tzai Li, Keduo Qian, Kenneth F. Bastow, Kuo Hsiung Lee, Tian Shung Wu

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Two new saponins, panajaponol (1) and pseudoginsenoside RT1 butyl ester (2), together with 35 known compounds (3-37), were isolated from the roots of Panax japonicus var. major. The structures of 1 and 2 were elucidated on the basis of spectroscopic analysis and chemical methods. Furthermore, a LC-MS/MS method was developed for confirming 2, 3, and 8 as natural compounds containing a butyl ester group. This method should be useful for distinguishing between minor natural and artifactual compounds in Panax species. Moreover, compounds 3, 6, 8, 9, 11, 13, and 15 exhibited strong inhibition of superoxide anion generation and elastase release by human neutrophils in response to formyl-l-methionyl-l-leucyl-l-phenylalanine/cytochalasin B (fMLP/CB), with IC50 values ranging from 0.78 to 43.6 μM. In addition, 1 showed greater than 2- to 3-fold selective cytotoxic activity against KB and DU145 cancer cell lines.

Original languageEnglish
Pages (from-to)796-802
Number of pages7
JournalJournal of Natural Products
Volume74
Issue number4
DOIs
Publication statusPublished - 2011 Apr 25

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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