Bioassay-guided purification and identification of PPARα/γ agonists from Chlorella sorokiniana

Yu Cheng Chou, Ekambaranellore Prakash, Chien Fu Huang, Tzu Wen Lien, Xin Chen, Ih Jen Su, Yu Sheng Chao, Hsing Pang Hsieh, John Tsu An Hsu

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

This study isolated agonists of peroxisome proliferator activated receptors (PPARs) from the green algae Chlorella sorokiniana, using a bioassay-guided purification strategy. PPARs are widely recognized as the molecular drug targets for many diseases including hyperglycemia, diabetes, obesity and cancer. Two independent bioassays were developed. The first is the scintillation proximity assay, a ligand binding assay. The other is the cell-based transcriptional activation assay which uses the Dual-Luciferase® reporter system as the reporter gene under the control of the PPAR response element. Using these two assays, a PPARγ-active fraction, CE 3-3, was obtained from C. sorokiniana extracts, which was also able to activate PPARα-mediated gene expression. To elucidate the active ingredients in the CE 3-3 fraction, GC-MS analysis was employed. The results showed that the CE 3-3 fraction consisted of at least ten fatty acids (FAs). The bioactivities of several of the individual FAs were evaluated for their PPARγ activity and the results showed that linolenic acid and linoleic acid were the most potent FAs tested. Our studies indicate that Chlorella sorokiniana could have potential health benefits through the dual activation of PPARα/γ via its unique FA constituents.

Original languageEnglish
Pages (from-to)605-613
Number of pages9
JournalPhytotherapy Research
Volume22
Issue number5
DOIs
Publication statusPublished - 2008 May

All Science Journal Classification (ASJC) codes

  • Pharmacology

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