Bioaugmentation and biostimulation for enhanced TPHd biodegradation in diesel-contaminated soil

Pao Wen G. Liu, Liang-Ming Whang, M. J. Yang, Y. C. Fan, L. H. Chang, S. L. Su, T. C. Chang, S. S. Cheng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Contamination with petroleum hydrocarbon compounds in soil and groundwater draws attention regarding environmental and healthy issues, resulting in an increased interest in developing innovative and sound technologies for its remediation. Bioremediation of petroleum hydrocarbons has been proposed as an effective, economic, and environmentally-friendly technology, although the presence of contaminantdegrading bacteria and bioavailability of hydrophobic organic compounds (HOCs) to microorganisms could be limiting factors during the biodegradation process. This study investigated the potential application of bioaugmentation and biosurfactants (rhamnolipid) for enhanced biodegradation of diesel-contaminated soil with a series of bench-scale experiments. Measurements of soil total petroleum hydrocarbon-diesel (TPHd) indicated that all eight tested biopiles provided enhanced biodegradation abilities, with respect to both diesel removal efficiency and biodegradation rate, in soil samples contaminated with diesel at a concentration of 20,000 mg-TPHd/kg soil. Molecular methods, including microarray hybridization, terminal restriction fragment length polymorphism (T-RFLP), and denaturing gradient gel electrophoresis (DGGE), were applied to monitor the microbial population dynamics of augmented diesel-degrading bacteria in tested biopiles. The results of microbial community analyses confirmed the application of bioaugmentation in biopiles conducted in this study would be a beneficial measure to increase the microbial diversity of diesel-degrading bacteria and the diesel biodegradation rate.

Original languageEnglish
Title of host publicationBattelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007
Pages372-380
Number of pages9
Publication statusPublished - 2007 Dec 1
Event9th International In Situ and On-Site Bioremediation Symposium 2007 - Baltimore, MD, United States
Duration: 2007 May 72007 May 10

Publication series

NameBattelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007
Volume1

Other

Other9th International In Situ and On-Site Bioremediation Symposium 2007
CountryUnited States
CityBaltimore, MD
Period07-05-0707-05-10

All Science Journal Classification (ASJC) codes

  • Ecological Modelling
  • Environmental Engineering
  • Bioengineering
  • Waste Management and Disposal

Fingerprint Dive into the research topics of 'Bioaugmentation and biostimulation for enhanced TPH<sup>d</sup> biodegradation in diesel-contaminated soil'. Together they form a unique fingerprint.

  • Cite this

    Liu, P. W. G., Whang, L-M., Yang, M. J., Fan, Y. C., Chang, L. H., Su, S. L., Chang, T. C., & Cheng, S. S. (2007). Bioaugmentation and biostimulation for enhanced TPHd biodegradation in diesel-contaminated soil. In Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007 (pp. 372-380). (Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007; Vol. 1).