Biochemical characterization of an acid phosphatase from thermus thermophilus

Sy Jye Tham, Ching Dong Chang, Hao-Jen Huang, Yueh Feng Lee, Tze Sing Huang, Ching-Chun Chang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A recombinant putative acid phosphatase from Thermus thermophilus was expressed and purified from Escherichia coli. The recombinant phosphatase displayed activities in a broad range of temperature, from 40 to 90 °C, with optimal temperature at 70 °C. In addition, the recombinant enzyme had activities in a wide range of pH, from 3.6 to 9.1, with optimal pH at 6 in acetate buffer and with optimal pH at 6.5 in Hepes buffer. Furthermore, it showed significant thermal stability and still possessed 44% residual activity after 70 °C treatment for 15 min. Moreover, the recombinant phosphatase showed broad substrates specificities for monophosphate esters, p-nitrophenyl phosphate (pNPP) being the most preferred substrate, and it was able to resist inhibition by sodium tartrate. Additionally, the recombinant protein formed stable oligomer under partially denatured conditions and required calcium ions for enzymic activity.

Original languageEnglish
Pages (from-to)727-735
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume74
Issue number4
DOIs
Publication statusPublished - 2010 May 3

Fingerprint

Thermus thermophilus
Acid Phosphatase
Phosphoric Monoester Hydrolases
Buffers
Enzyme activity
Substrates
Recombinant Proteins
Oligomers
Escherichia coli
Esters
Acetates
Temperature
Thermodynamic stability
Ions
Substrate Specificity
Calcium
Hot Temperature
Enzymes
nitrophenylphosphate
tartaric acid

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Tham, Sy Jye ; Chang, Ching Dong ; Huang, Hao-Jen ; Lee, Yueh Feng ; Huang, Tze Sing ; Chang, Ching-Chun. / Biochemical characterization of an acid phosphatase from thermus thermophilus. In: Bioscience, Biotechnology and Biochemistry. 2010 ; Vol. 74, No. 4. pp. 727-735.
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Biochemical characterization of an acid phosphatase from thermus thermophilus. / Tham, Sy Jye; Chang, Ching Dong; Huang, Hao-Jen; Lee, Yueh Feng; Huang, Tze Sing; Chang, Ching-Chun.

In: Bioscience, Biotechnology and Biochemistry, Vol. 74, No. 4, 03.05.2010, p. 727-735.

Research output: Contribution to journalArticle

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