TY - JOUR
T1 - Biodegradation of diesel-contaminated soil
T2 - A soil column study
AU - Liu, Pao Wen Grace
AU - Whang, Liang Ming
AU - Yang, Ming Chieh
AU - Cheng, Sheng Shung
N1 - Funding Information:
The authors like to give our appreciation to Professor Jo-Shu Chang, Department of Chemical Engineering, National Cheng Kung University, for their contribution on biosurfactant production. Also thanks to Professor Chiu-Chung Young, Department of Soil and Environmental Sciences, National Chung Hsing University, for the provision of the bacterial isolates. Funding of this study was provided by the Ministry of Economic Affairs, R.O.C. under contract no. 95-EC-17-A-10-S1-0013.
PY - 2008/9
Y1 - 2008/9
N2 - This study simulated in situ bioremediation for diesel-contaminated soil by a column operation. Several bioremediation approaches were conducted to examine the feasibility. Four lab-scale soil columns were operated specific to the following approaches: nutrient enhancement (NE), bioaugmentation (BA), biostimulation (BS), and sterilized soil (SS). Within 330 days of operation, the residual total petroleum hydrocarbon-diesel (TPHd) was degraded from 10,290 mg TPHd/dry soil to 3026, 1851, 4105, and 6506 mg TPHd/kg dry soil by Columns NE, BA, BS, and SS, respectively. The diesel-degradation efficiency was 67%, 80%, 45%, and 24%, accordingly. Microbial diversity was monitored with molecular biotechnology DGGE. It was found that introduced bacteria CC-CF3 and CC-JG39 become undetectable after 90 days of operation, but another introduced bacteria, CC-RS1, evidently remained with small signals during the last time stage of operation.
AB - This study simulated in situ bioremediation for diesel-contaminated soil by a column operation. Several bioremediation approaches were conducted to examine the feasibility. Four lab-scale soil columns were operated specific to the following approaches: nutrient enhancement (NE), bioaugmentation (BA), biostimulation (BS), and sterilized soil (SS). Within 330 days of operation, the residual total petroleum hydrocarbon-diesel (TPHd) was degraded from 10,290 mg TPHd/dry soil to 3026, 1851, 4105, and 6506 mg TPHd/kg dry soil by Columns NE, BA, BS, and SS, respectively. The diesel-degradation efficiency was 67%, 80%, 45%, and 24%, accordingly. Microbial diversity was monitored with molecular biotechnology DGGE. It was found that introduced bacteria CC-CF3 and CC-JG39 become undetectable after 90 days of operation, but another introduced bacteria, CC-RS1, evidently remained with small signals during the last time stage of operation.
UR - http://www.scopus.com/inward/record.url?scp=56949088015&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=56949088015&partnerID=8YFLogxK
U2 - 10.1016/j.jcice.2008.03.006
DO - 10.1016/j.jcice.2008.03.006
M3 - Article
AN - SCOPUS:56949088015
SN - 0368-1653
VL - 39
SP - 419
EP - 428
JO - Journal of the Chinese Institute of Chemical Engineers
JF - Journal of the Chinese Institute of Chemical Engineers
IS - 5
ER -