Biodiesel production by enzymatic transesterification catalyzed by Burkholderia lipase immobilized on hydrophobic magnetic particles

Chien Hung Liu, Chien Chang Huang, Yao Wen Wang, Duu Jong Lee, Jo Shu Chang

Research output: Contribution to journalArticlepeer-review

74 Citations (Scopus)

Abstract

Biodiesel is a promising substitute for petroleum diesel, and has been commercialized and utilized in many countries. Conventional chemical or physical methods used for biodiesel production face the drawbacks of high energy consumption or intensive use of chemicals. In contrast, using lipase-catalyzed transesterification for biodiesel synthesis is clean, effective, and water tolerance. Therefore, in this work, a self-developed Burkholderia lipase was immobilized onto hydrophobic magnetic particles (HMPs) for biodiesel production. Transesterification with the immobilized lipase could be repeatedly carried out six times without severe activity loss. The optimal conditions for the enzymatic transesterification were identified as: room temperature, 200. rpm agitation, 10% water content, and a methanol-to-oil molar ratio of 4:1. Under these conditions, the conversion of oil to fatty acid methyl esters (FAMEs) reached nearly 70% within 12. h, giving a biodiesel production rate of 43.5. g/L/h.

Original languageEnglish
Pages (from-to)41-46
Number of pages6
JournalApplied Energy
Volume100
DOIs
Publication statusPublished - 2012 Dec

All Science Journal Classification (ASJC) codes

  • Building and Construction
  • Energy(all)
  • Mechanical Engineering
  • Management, Monitoring, Policy and Law

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