Biogenesis of podosome rosettes through fission

Szu Lin Kuo, Chien Lin Chen, Yi Ru Pan, Wen-Tai Chiu, Hong Chen Chen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Podosomes are dynamic actin-based membrane protrusions that are important for extracellular matrix degradation and invasive cell motility. Individual podosomes are often found to organize into large rosette-like structures in some types of cells, such as osteoclasts, endothelial cells, Src-transformed fibroblasts, and certain highly invasive cancer cells. In this study, we show that new podosome rosettes arise through one of two mechanisms; de novo assembly or fission of a pre-existing podosome rosette in Src-transformed fibroblasts. Fission is a more efficient way than de novo assembly to generate new podosome rosettes in these cells. Podosome rosettes undergoing fission possess higher motility and a stronger matrix-degrading capability. Podosome rosette fission may be the result of polarized myosin II-mediated contractility of these structures, which is coordinately regulated by myosin light chain kinase and Rho-associated kinase II. Collectively, this study unveils a previously unknown mechanism - fission for the biogenesis of podosome rosettes.

Original languageEnglish
Article number524
JournalScientific reports
Volume8
Issue number1
DOIs
Publication statusPublished - 2018 Dec 1

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Fibroblasts
Myosin Type II
Myosin-Light-Chain Kinase
rho-Associated Kinases
Podosomes
Osteoclasts
Cell Movement
Extracellular Matrix
Actins
Endothelial Cells
Membranes
Neoplasms

All Science Journal Classification (ASJC) codes

  • General

Cite this

Kuo, Szu Lin ; Chen, Chien Lin ; Pan, Yi Ru ; Chiu, Wen-Tai ; Chen, Hong Chen. / Biogenesis of podosome rosettes through fission. In: Scientific reports. 2018 ; Vol. 8, No. 1.
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Biogenesis of podosome rosettes through fission. / Kuo, Szu Lin; Chen, Chien Lin; Pan, Yi Ru; Chiu, Wen-Tai; Chen, Hong Chen.

In: Scientific reports, Vol. 8, No. 1, 524, 01.12.2018.

Research output: Contribution to journalArticle

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