Biogenesis of podosome rosettes through fission

Szu Lin Kuo, Chien Lin Chen, Yi Ru Pan, Wen Tai Chiu, Hong Chen Chen

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)


Podosomes are dynamic actin-based membrane protrusions that are important for extracellular matrix degradation and invasive cell motility. Individual podosomes are often found to organize into large rosette-like structures in some types of cells, such as osteoclasts, endothelial cells, Src-transformed fibroblasts, and certain highly invasive cancer cells. In this study, we show that new podosome rosettes arise through one of two mechanisms; de novo assembly or fission of a pre-existing podosome rosette in Src-transformed fibroblasts. Fission is a more efficient way than de novo assembly to generate new podosome rosettes in these cells. Podosome rosettes undergoing fission possess higher motility and a stronger matrix-degrading capability. Podosome rosette fission may be the result of polarized myosin II-mediated contractility of these structures, which is coordinately regulated by myosin light chain kinase and Rho-associated kinase II. Collectively, this study unveils a previously unknown mechanism - fission for the biogenesis of podosome rosettes.

Original languageEnglish
Article number524
JournalScientific reports
Issue number1
Publication statusPublished - 2018 Dec 1

All Science Journal Classification (ASJC) codes

  • General


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