Biohydrogen from cellulosic feedstock

Dilution-to-stimulation approach

Kuo Ling Ho, Duu Jong Lee, Ay Su, Jo-Shu Chang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Biohydrogen produced from cellulosic feedstock is a promising candidate as a clean energy carrier to meet future energy needs. This work identified a functional consortium that can produce biohydrogen from three cellulosic feedstocks, the Whatman No. 2 filter, xylan, and Chlorella cells, using a novel dilution-to-stimulation approach. By serially diluting an anaerobic inoculum, biohydrogen production increased, peaked at a specific dilution ratio, and then declined. With filter paper tests at 10 -9 dilution, the functional consortium was mainly composed of Clostridium sp. clone UC61. At dilutions greater than 10 -9, the presence of other heterotrophic strains diminished the ability of consortium to produce hydrogen. In the xylan tests, Clostridium sp. clone UC61, Clostridium sp. BN2, and Bacteroides species comprised the functional consortium. Klebsiella sp. competed with Clostridium sp. for carbon and decreased hydrogen production. In the Chlorella cells tests, Bacillus, Acinetobacter, Elusimicrobium, and Clostridium comprised the functional consortium. The loss of Bacillus and Acinetobacter species under high dilution deteriorated the synergistic relationships among different strains. The proposed dilution-to-stimulation approach is effective for isolating functional strains for simultaneous hydrolysis and hydrogen production by cellulosic materials.

Original languageEnglish
Pages (from-to)15582-15587
Number of pages6
JournalInternational Journal of Hydrogen Energy
Volume37
Issue number20
DOIs
Publication statusPublished - 2012 Oct 1

Fingerprint

Clostridium
stimulation
Feedstocks
Dilution
dilution
Chlorella
Bacillus
hydrogen production
Bacilli
Hydrogen production
inoculum
Klebsiella
clean energy
filters
cells
hydrolysis
Hydrolysis
Hydrogen
Carbon
carbon

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

Ho, Kuo Ling ; Lee, Duu Jong ; Su, Ay ; Chang, Jo-Shu. / Biohydrogen from cellulosic feedstock : Dilution-to-stimulation approach. In: International Journal of Hydrogen Energy. 2012 ; Vol. 37, No. 20. pp. 15582-15587.
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Biohydrogen from cellulosic feedstock : Dilution-to-stimulation approach. / Ho, Kuo Ling; Lee, Duu Jong; Su, Ay; Chang, Jo-Shu.

In: International Journal of Hydrogen Energy, Vol. 37, No. 20, 01.10.2012, p. 15582-15587.

Research output: Contribution to journalArticle

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