Biohydrogen production with fixed-bed bioreactors

Jo-Shu Chang, Kuo Shing Lee, Pin Jei Lin

Research output: Contribution to journalConference article

312 Citations (Scopus)

Abstract

An investigation on anaerobic hydrogen production was conducted in fixed-bed bioreactors containing hydrogen-producing bacteria originated from domestic sewage sludge. Three porous materials, loofah sponge (LS), expanded clay (EC) and activated carbon (AC), were used as the support matrix to allow retention of the hydrogen-producing bacteria within the fixed-bed bioreactors. The carriers were assessed for their effectiveness in biofilm formation and hydrogen production in batch and continuous modes. It was found that LS was inefficient for biomass immobilization, while EC and AC exhibited better biomass yields. The fixed-bed reactors packed with EC or AC (denote as EC or AC reactors) were thus used for continuous hydrogen fermentation at a hydraulic retention time (HRT) of 0.5-5 h. Sucrose was utilized as the major carbon source. With a sucrose concentration of ca. 20 g COD/l in the feed, the EC reactor (workingvolume = 300 ml) was able to produce H2 at an optimal rate of 0.415 l/h/l at HRT = 2 h. In contrast, the AC reactor (300 ml in volume) exhibited a better hydrogen production rate of 1.32 l/h/l, which occurred at HRT = 1 h. When the AC reactor was scaled up to 3 l, the hydrogen production rate was nearly 0.53-0.68 l/h/l for HRT = 1-3 h, but after a short thermal treatment (75°C, 1 h) the rate rose to ca. 1.21 l/h/l at HRT = 1 h. The biogas produced with EC and AC reactors typically contained 25-35% of H2 and the rest was mainly CO2, while production of methane was negligible (less than 0.1%). During the efficient hydrogen production stage, the major soluble metabolite was butyric acid, followed by propionic acid, acetic acid, and ethanol.

Original languageEnglish
Pages (from-to)1167-1174
Number of pages8
JournalInternational Journal of Hydrogen Energy
Volume27
Issue number11-12
DOIs
Publication statusPublished - 2002 Nov 1
EventBiohydrogen 2002 (BIO-H2) - Ede, Netherlands
Duration: 2002 Apr 212002 Apr 21

Fingerprint

bioreactors
activated carbon
Bioreactors
Activated carbon
beds
clays
hydrogen production
Hydrogen production
Clay
hydraulics
reactors
Hydraulics
sucrose
Sugar (sucrose)
biomass
Hydrogen
bacteria
Bacteria
Biomass
hydrogen

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

Chang, Jo-Shu ; Lee, Kuo Shing ; Lin, Pin Jei. / Biohydrogen production with fixed-bed bioreactors. In: International Journal of Hydrogen Energy. 2002 ; Vol. 27, No. 11-12. pp. 1167-1174.
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abstract = "An investigation on anaerobic hydrogen production was conducted in fixed-bed bioreactors containing hydrogen-producing bacteria originated from domestic sewage sludge. Three porous materials, loofah sponge (LS), expanded clay (EC) and activated carbon (AC), were used as the support matrix to allow retention of the hydrogen-producing bacteria within the fixed-bed bioreactors. The carriers were assessed for their effectiveness in biofilm formation and hydrogen production in batch and continuous modes. It was found that LS was inefficient for biomass immobilization, while EC and AC exhibited better biomass yields. The fixed-bed reactors packed with EC or AC (denote as EC or AC reactors) were thus used for continuous hydrogen fermentation at a hydraulic retention time (HRT) of 0.5-5 h. Sucrose was utilized as the major carbon source. With a sucrose concentration of ca. 20 g COD/l in the feed, the EC reactor (workingvolume = 300 ml) was able to produce H2 at an optimal rate of 0.415 l/h/l at HRT = 2 h. In contrast, the AC reactor (300 ml in volume) exhibited a better hydrogen production rate of 1.32 l/h/l, which occurred at HRT = 1 h. When the AC reactor was scaled up to 3 l, the hydrogen production rate was nearly 0.53-0.68 l/h/l for HRT = 1-3 h, but after a short thermal treatment (75°C, 1 h) the rate rose to ca. 1.21 l/h/l at HRT = 1 h. The biogas produced with EC and AC reactors typically contained 25-35{\%} of H2 and the rest was mainly CO2, while production of methane was negligible (less than 0.1{\%}). During the efficient hydrogen production stage, the major soluble metabolite was butyric acid, followed by propionic acid, acetic acid, and ethanol.",
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Biohydrogen production with fixed-bed bioreactors. / Chang, Jo-Shu; Lee, Kuo Shing; Lin, Pin Jei.

In: International Journal of Hydrogen Energy, Vol. 27, No. 11-12, 01.11.2002, p. 1167-1174.

Research output: Contribution to journalConference article

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T1 - Biohydrogen production with fixed-bed bioreactors

AU - Chang, Jo-Shu

AU - Lee, Kuo Shing

AU - Lin, Pin Jei

PY - 2002/11/1

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N2 - An investigation on anaerobic hydrogen production was conducted in fixed-bed bioreactors containing hydrogen-producing bacteria originated from domestic sewage sludge. Three porous materials, loofah sponge (LS), expanded clay (EC) and activated carbon (AC), were used as the support matrix to allow retention of the hydrogen-producing bacteria within the fixed-bed bioreactors. The carriers were assessed for their effectiveness in biofilm formation and hydrogen production in batch and continuous modes. It was found that LS was inefficient for biomass immobilization, while EC and AC exhibited better biomass yields. The fixed-bed reactors packed with EC or AC (denote as EC or AC reactors) were thus used for continuous hydrogen fermentation at a hydraulic retention time (HRT) of 0.5-5 h. Sucrose was utilized as the major carbon source. With a sucrose concentration of ca. 20 g COD/l in the feed, the EC reactor (workingvolume = 300 ml) was able to produce H2 at an optimal rate of 0.415 l/h/l at HRT = 2 h. In contrast, the AC reactor (300 ml in volume) exhibited a better hydrogen production rate of 1.32 l/h/l, which occurred at HRT = 1 h. When the AC reactor was scaled up to 3 l, the hydrogen production rate was nearly 0.53-0.68 l/h/l for HRT = 1-3 h, but after a short thermal treatment (75°C, 1 h) the rate rose to ca. 1.21 l/h/l at HRT = 1 h. The biogas produced with EC and AC reactors typically contained 25-35% of H2 and the rest was mainly CO2, while production of methane was negligible (less than 0.1%). During the efficient hydrogen production stage, the major soluble metabolite was butyric acid, followed by propionic acid, acetic acid, and ethanol.

AB - An investigation on anaerobic hydrogen production was conducted in fixed-bed bioreactors containing hydrogen-producing bacteria originated from domestic sewage sludge. Three porous materials, loofah sponge (LS), expanded clay (EC) and activated carbon (AC), were used as the support matrix to allow retention of the hydrogen-producing bacteria within the fixed-bed bioreactors. The carriers were assessed for their effectiveness in biofilm formation and hydrogen production in batch and continuous modes. It was found that LS was inefficient for biomass immobilization, while EC and AC exhibited better biomass yields. The fixed-bed reactors packed with EC or AC (denote as EC or AC reactors) were thus used for continuous hydrogen fermentation at a hydraulic retention time (HRT) of 0.5-5 h. Sucrose was utilized as the major carbon source. With a sucrose concentration of ca. 20 g COD/l in the feed, the EC reactor (workingvolume = 300 ml) was able to produce H2 at an optimal rate of 0.415 l/h/l at HRT = 2 h. In contrast, the AC reactor (300 ml in volume) exhibited a better hydrogen production rate of 1.32 l/h/l, which occurred at HRT = 1 h. When the AC reactor was scaled up to 3 l, the hydrogen production rate was nearly 0.53-0.68 l/h/l for HRT = 1-3 h, but after a short thermal treatment (75°C, 1 h) the rate rose to ca. 1.21 l/h/l at HRT = 1 h. The biogas produced with EC and AC reactors typically contained 25-35% of H2 and the rest was mainly CO2, while production of methane was negligible (less than 0.1%). During the efficient hydrogen production stage, the major soluble metabolite was butyric acid, followed by propionic acid, acetic acid, and ethanol.

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DO - 10.1016/S0360-3199(02)00130-1

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JO - International Journal of Hydrogen Energy

JF - International Journal of Hydrogen Energy

SN - 0360-3199

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