Biopile study of the effects of bioaugmentation and biostimulation on bioremediation of TPH-contaminated soil

Po Tseng Pan, Sheng Shung Cheng, Chun Hsuan Gaop, Tsung Lin Hsieh, Li Hsin Chang, Liang-Ming Whang, Tsung Chung Chang, Pao Wen Liu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Contamination of total petroleum hydrocarbons (TPH) has caused critical environmental and health defects. Laboratory microcosms were used to measure the change in TPH concentration and the accompanying changes in microbial diversity with time. During the experiments, soil samples were collected and analyzed periodically to determine the amount of residual TPH and evaluate the effects of amendments on microbial diversity. A recently TPH-contaminated soil site has investigated to apply different enhanced bioremediation process with lab-scale of 0.0015m3 biopiles. Conceptual design of five biopiles was conducted to provide: (1).non-agitation biopile with the indigenous microbes as Natural Attenuation test, NA, (2).well-mixing biopile with the indigenous microbes as control test, CT, (3).bioaugmentation with five types of enrichment culture of TPH-degrading bacteria, BAT, (4).biostimulation with biosurfactant (rhamnolipid) to emulsify the entrapped diesel adsorbed onto the soil pore, BS. (5).combined bioaugmentation and biostimulation with TPH-degrading bacteria and biosurfactant as BAS test. Besides NA biopile, periodic land-farming procedure was employed to these four biopiles(CTinverted commas BATinverted commas BSinverted commas BAS). Weekly turn over mixing and water spray could keep 0.0015m3 of loamy sand soil with enough oxygen content above 10%-15% and moisture about 20%. The original total petroleum hydrocarbon (TPH C10-C40) was about 1,4032 mg TPH/ kg dry soil and the total indigenous microbial concentration was measured at the range of 3.0×104 CFU/g soil that meaned the biodegradation would be happened. Therefore, the TPH removal efficiencies (Day 112) achieved at different levels in these five biopiles, NA=24.8%, CT=62.7%, BAT=67.2%, BS=77.2%, BAS=72.7%. Bioremediation performance was promoted by the bioaugmentation and the biosurfactant addition (BS). Before 35 days, the first order reaction rate constant K values (day-1) was evaluated as BAS(0.0329)> BAT(0.0189) >BS(0.0151)>CT(0.0095)>NA(0.0009). However, the slowing of degradation rate during the later stage could be attributed to the properties of the remaining hydrocarbons and environmental conditions. This increase of polar fraction to the results of biomass formation associated with the biodegration of hydrocarbons.

Original languageEnglish
Title of host publicationIn Situ and On-Site Bioremediation-2009
Subtitle of host publicationProceedings of the 10th International In Situ and On-Site Bioremediation Symposium
Publication statusPublished - 2009 Nov 27
Event10th International In Situ and On-Site Bioremediation Symposium, In Situ and On-Site Bioremediation-2009 - Baltimore, MD, United States
Duration: 2009 May 52009 May 8

Publication series

NameIn Situ and On-Site Bioremediation-2009: Proceedings of the 10th International In Situ and On-Site Bioremediation Symposium

Other

Other10th International In Situ and On-Site Bioremediation Symposium, In Situ and On-Site Bioremediation-2009
CountryUnited States
CityBaltimore, MD
Period09-05-0509-05-08

Fingerprint

Environmental Biodegradation
Petroleum
petroleum hydrocarbon
Hydrocarbons
bioremediation
Soil
soil
effect
contaminated soil
hydrocarbon
bacterium
natural attenuation
loamy sand
Bacteria
Environmental Health
microcosm
reaction rate
diesel
defect
Agriculture

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Management, Monitoring, Policy and Law
  • Waste Management and Disposal

Cite this

Pan, P. T., Cheng, S. S., Gaop, C. H., Hsieh, T. L., Chang, L. H., Whang, L-M., ... Liu, P. W. (2009). Biopile study of the effects of bioaugmentation and biostimulation on bioremediation of TPH-contaminated soil. In In Situ and On-Site Bioremediation-2009: Proceedings of the 10th International In Situ and On-Site Bioremediation Symposium (In Situ and On-Site Bioremediation-2009: Proceedings of the 10th International In Situ and On-Site Bioremediation Symposium).
Pan, Po Tseng ; Cheng, Sheng Shung ; Gaop, Chun Hsuan ; Hsieh, Tsung Lin ; Chang, Li Hsin ; Whang, Liang-Ming ; Chang, Tsung Chung ; Liu, Pao Wen. / Biopile study of the effects of bioaugmentation and biostimulation on bioremediation of TPH-contaminated soil. In Situ and On-Site Bioremediation-2009: Proceedings of the 10th International In Situ and On-Site Bioremediation Symposium. 2009. (In Situ and On-Site Bioremediation-2009: Proceedings of the 10th International In Situ and On-Site Bioremediation Symposium).
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title = "Biopile study of the effects of bioaugmentation and biostimulation on bioremediation of TPH-contaminated soil",
abstract = "Contamination of total petroleum hydrocarbons (TPH) has caused critical environmental and health defects. Laboratory microcosms were used to measure the change in TPH concentration and the accompanying changes in microbial diversity with time. During the experiments, soil samples were collected and analyzed periodically to determine the amount of residual TPH and evaluate the effects of amendments on microbial diversity. A recently TPH-contaminated soil site has investigated to apply different enhanced bioremediation process with lab-scale of 0.0015m3 biopiles. Conceptual design of five biopiles was conducted to provide: (1).non-agitation biopile with the indigenous microbes as Natural Attenuation test, NA, (2).well-mixing biopile with the indigenous microbes as control test, CT, (3).bioaugmentation with five types of enrichment culture of TPH-degrading bacteria, BAT, (4).biostimulation with biosurfactant (rhamnolipid) to emulsify the entrapped diesel adsorbed onto the soil pore, BS. (5).combined bioaugmentation and biostimulation with TPH-degrading bacteria and biosurfactant as BAS test. Besides NA biopile, periodic land-farming procedure was employed to these four biopiles(CTinverted commas BATinverted commas BSinverted commas BAS). Weekly turn over mixing and water spray could keep 0.0015m3 of loamy sand soil with enough oxygen content above 10{\%}-15{\%} and moisture about 20{\%}. The original total petroleum hydrocarbon (TPH C10-C40) was about 1,4032 mg TPH/ kg dry soil and the total indigenous microbial concentration was measured at the range of 3.0×104 CFU/g soil that meaned the biodegradation would be happened. Therefore, the TPH removal efficiencies (Day 112) achieved at different levels in these five biopiles, NA=24.8{\%}, CT=62.7{\%}, BAT=67.2{\%}, BS=77.2{\%}, BAS=72.7{\%}. Bioremediation performance was promoted by the bioaugmentation and the biosurfactant addition (BS). Before 35 days, the first order reaction rate constant K values (day-1) was evaluated as BAS(0.0329)> BAT(0.0189) >BS(0.0151)>CT(0.0095)>NA(0.0009). However, the slowing of degradation rate during the later stage could be attributed to the properties of the remaining hydrocarbons and environmental conditions. This increase of polar fraction to the results of biomass formation associated with the biodegration of hydrocarbons.",
author = "Pan, {Po Tseng} and Cheng, {Sheng Shung} and Gaop, {Chun Hsuan} and Hsieh, {Tsung Lin} and Chang, {Li Hsin} and Liang-Ming Whang and Chang, {Tsung Chung} and Liu, {Pao Wen}",
year = "2009",
month = "11",
day = "27",
language = "English",
isbn = "9780981973012",
series = "In Situ and On-Site Bioremediation-2009: Proceedings of the 10th International In Situ and On-Site Bioremediation Symposium",
booktitle = "In Situ and On-Site Bioremediation-2009",

}

Pan, PT, Cheng, SS, Gaop, CH, Hsieh, TL, Chang, LH, Whang, L-M, Chang, TC & Liu, PW 2009, Biopile study of the effects of bioaugmentation and biostimulation on bioremediation of TPH-contaminated soil. in In Situ and On-Site Bioremediation-2009: Proceedings of the 10th International In Situ and On-Site Bioremediation Symposium. In Situ and On-Site Bioremediation-2009: Proceedings of the 10th International In Situ and On-Site Bioremediation Symposium, 10th International In Situ and On-Site Bioremediation Symposium, In Situ and On-Site Bioremediation-2009, Baltimore, MD, United States, 09-05-05.

Biopile study of the effects of bioaugmentation and biostimulation on bioremediation of TPH-contaminated soil. / Pan, Po Tseng; Cheng, Sheng Shung; Gaop, Chun Hsuan; Hsieh, Tsung Lin; Chang, Li Hsin; Whang, Liang-Ming; Chang, Tsung Chung; Liu, Pao Wen.

In Situ and On-Site Bioremediation-2009: Proceedings of the 10th International In Situ and On-Site Bioremediation Symposium. 2009. (In Situ and On-Site Bioremediation-2009: Proceedings of the 10th International In Situ and On-Site Bioremediation Symposium).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Biopile study of the effects of bioaugmentation and biostimulation on bioremediation of TPH-contaminated soil

AU - Pan, Po Tseng

AU - Cheng, Sheng Shung

AU - Gaop, Chun Hsuan

AU - Hsieh, Tsung Lin

AU - Chang, Li Hsin

AU - Whang, Liang-Ming

AU - Chang, Tsung Chung

AU - Liu, Pao Wen

PY - 2009/11/27

Y1 - 2009/11/27

N2 - Contamination of total petroleum hydrocarbons (TPH) has caused critical environmental and health defects. Laboratory microcosms were used to measure the change in TPH concentration and the accompanying changes in microbial diversity with time. During the experiments, soil samples were collected and analyzed periodically to determine the amount of residual TPH and evaluate the effects of amendments on microbial diversity. A recently TPH-contaminated soil site has investigated to apply different enhanced bioremediation process with lab-scale of 0.0015m3 biopiles. Conceptual design of five biopiles was conducted to provide: (1).non-agitation biopile with the indigenous microbes as Natural Attenuation test, NA, (2).well-mixing biopile with the indigenous microbes as control test, CT, (3).bioaugmentation with five types of enrichment culture of TPH-degrading bacteria, BAT, (4).biostimulation with biosurfactant (rhamnolipid) to emulsify the entrapped diesel adsorbed onto the soil pore, BS. (5).combined bioaugmentation and biostimulation with TPH-degrading bacteria and biosurfactant as BAS test. Besides NA biopile, periodic land-farming procedure was employed to these four biopiles(CTinverted commas BATinverted commas BSinverted commas BAS). Weekly turn over mixing and water spray could keep 0.0015m3 of loamy sand soil with enough oxygen content above 10%-15% and moisture about 20%. The original total petroleum hydrocarbon (TPH C10-C40) was about 1,4032 mg TPH/ kg dry soil and the total indigenous microbial concentration was measured at the range of 3.0×104 CFU/g soil that meaned the biodegradation would be happened. Therefore, the TPH removal efficiencies (Day 112) achieved at different levels in these five biopiles, NA=24.8%, CT=62.7%, BAT=67.2%, BS=77.2%, BAS=72.7%. Bioremediation performance was promoted by the bioaugmentation and the biosurfactant addition (BS). Before 35 days, the first order reaction rate constant K values (day-1) was evaluated as BAS(0.0329)> BAT(0.0189) >BS(0.0151)>CT(0.0095)>NA(0.0009). However, the slowing of degradation rate during the later stage could be attributed to the properties of the remaining hydrocarbons and environmental conditions. This increase of polar fraction to the results of biomass formation associated with the biodegration of hydrocarbons.

AB - Contamination of total petroleum hydrocarbons (TPH) has caused critical environmental and health defects. Laboratory microcosms were used to measure the change in TPH concentration and the accompanying changes in microbial diversity with time. During the experiments, soil samples were collected and analyzed periodically to determine the amount of residual TPH and evaluate the effects of amendments on microbial diversity. A recently TPH-contaminated soil site has investigated to apply different enhanced bioremediation process with lab-scale of 0.0015m3 biopiles. Conceptual design of five biopiles was conducted to provide: (1).non-agitation biopile with the indigenous microbes as Natural Attenuation test, NA, (2).well-mixing biopile with the indigenous microbes as control test, CT, (3).bioaugmentation with five types of enrichment culture of TPH-degrading bacteria, BAT, (4).biostimulation with biosurfactant (rhamnolipid) to emulsify the entrapped diesel adsorbed onto the soil pore, BS. (5).combined bioaugmentation and biostimulation with TPH-degrading bacteria and biosurfactant as BAS test. Besides NA biopile, periodic land-farming procedure was employed to these four biopiles(CTinverted commas BATinverted commas BSinverted commas BAS). Weekly turn over mixing and water spray could keep 0.0015m3 of loamy sand soil with enough oxygen content above 10%-15% and moisture about 20%. The original total petroleum hydrocarbon (TPH C10-C40) was about 1,4032 mg TPH/ kg dry soil and the total indigenous microbial concentration was measured at the range of 3.0×104 CFU/g soil that meaned the biodegradation would be happened. Therefore, the TPH removal efficiencies (Day 112) achieved at different levels in these five biopiles, NA=24.8%, CT=62.7%, BAT=67.2%, BS=77.2%, BAS=72.7%. Bioremediation performance was promoted by the bioaugmentation and the biosurfactant addition (BS). Before 35 days, the first order reaction rate constant K values (day-1) was evaluated as BAS(0.0329)> BAT(0.0189) >BS(0.0151)>CT(0.0095)>NA(0.0009). However, the slowing of degradation rate during the later stage could be attributed to the properties of the remaining hydrocarbons and environmental conditions. This increase of polar fraction to the results of biomass formation associated with the biodegration of hydrocarbons.

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M3 - Conference contribution

SN - 9780981973012

T3 - In Situ and On-Site Bioremediation-2009: Proceedings of the 10th International In Situ and On-Site Bioremediation Symposium

BT - In Situ and On-Site Bioremediation-2009

ER -

Pan PT, Cheng SS, Gaop CH, Hsieh TL, Chang LH, Whang L-M et al. Biopile study of the effects of bioaugmentation and biostimulation on bioremediation of TPH-contaminated soil. In In Situ and On-Site Bioremediation-2009: Proceedings of the 10th International In Situ and On-Site Bioremediation Symposium. 2009. (In Situ and On-Site Bioremediation-2009: Proceedings of the 10th International In Situ and On-Site Bioremediation Symposium).