TY - JOUR
T1 - Bioprocess development on microalgae-based CO2 fixation and bioethanol production using Scenedesmus obliquus CNW-N
AU - Ho, Shih Hsin
AU - Li, Po Jen
AU - Liu, Chen Chun
AU - Chang, Jo-Shu
PY - 2013/1/1
Y1 - 2013/1/1
N2 - A two-stage cultivation strategy was applied to achieve greater CO2 fixation and carbohydrate productivity with an indigenous microalga Scenedesmus obliquus CNW-N, which was first cultivated using a nutrient-rich medium to promote cell growth, and was then switched to a nutrient-deficient condition to trigger carbohydrate accumulation. The optimal biomass productivity, carbohydrate productivity, and CO2 fixation rate were 681.4, 352.9, and 1192.5mgL-1d-1, respectively, with a 51.8% carbohydrate content (based on dry weight). This performance is better than the results in most related studies. The microalgal carbohydrate was mainly composed of glucose, which accounts for nearly 80% of total sugars. Dilute acid hydrolysis with 2% H2SO4 can saccharify the wet microalgal biomass effectively, achieving a glucose yield of 96-98%. Using the acidic hydrolysate of the microalga as feedstock, the separate hydrolysis and fermentation (SHF) process gave an ethanol concentration of 8.55gL-1, representing a theoretical yield of nearly 99.8%.
AB - A two-stage cultivation strategy was applied to achieve greater CO2 fixation and carbohydrate productivity with an indigenous microalga Scenedesmus obliquus CNW-N, which was first cultivated using a nutrient-rich medium to promote cell growth, and was then switched to a nutrient-deficient condition to trigger carbohydrate accumulation. The optimal biomass productivity, carbohydrate productivity, and CO2 fixation rate were 681.4, 352.9, and 1192.5mgL-1d-1, respectively, with a 51.8% carbohydrate content (based on dry weight). This performance is better than the results in most related studies. The microalgal carbohydrate was mainly composed of glucose, which accounts for nearly 80% of total sugars. Dilute acid hydrolysis with 2% H2SO4 can saccharify the wet microalgal biomass effectively, achieving a glucose yield of 96-98%. Using the acidic hydrolysate of the microalga as feedstock, the separate hydrolysis and fermentation (SHF) process gave an ethanol concentration of 8.55gL-1, representing a theoretical yield of nearly 99.8%.
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U2 - 10.1016/j.biortech.2013.02.119
DO - 10.1016/j.biortech.2013.02.119
M3 - Article
C2 - 23566474
AN - SCOPUS:84883449660
SN - 0960-8524
VL - 145
SP - 142
EP - 149
JO - Bioresource Technology
JF - Bioresource Technology
ER -