Membrane introduction mass spectrometry with flow injection analysis (FIA) sampling has been utilized for on-line monitoring of the principal solution phase products of fermentation from the Klebsiella oxytoca microorganism. A flow injection sampling system was used to control delivery of fermentation broth or an external standard to the ion trap detector mass spectrometer. Analyte introduction occurred via a membrane probe in which the aqueous solutions flowed past a membrane located contiguous to the ion trap detector. Analytes which permeate the membrane were ionized by chemical ionization. The concentrations of the liquid phase products acetic acid, acetoin, 2,3-butanediol and ethanol were monitored as a function of time and quantified relative to external standard solutions. Off-line gas chromatography was utilized to test the accuracy of these measurements and excellent agreement was found. Products of fermentation by the organism Clostridium acetobutylicum have also been examined.
|Number of pages||12|
|Journal||Process Control and Quality|
|Publication status||Published - 1991 Feb 1|
All Science Journal Classification (ASJC) codes
- Control and Systems Engineering