Bioreactor monitoring using flow injection/membrane introduction mass spectrometry with an ion trap detector

Mark J. Hayward, Donald E. Riederer, Tapio Kotiaho, R. Graham Cooks, Glen D. Austin, Mei Jywan Syu, George T. Tsao

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

Membrane introduction mass spectrometry with flow injection analysis (FIA) sampling has been utilized for on-line monitoring of the principal solution phase products of fermentation from the Klebsiella oxytoca microorganism. A flow injection sampling system was used to control delivery of fermentation broth or an external standard to the ion trap detector mass spectrometer. Analyte introduction occurred via a membrane probe in which the aqueous solutions flowed past a membrane located contiguous to the ion trap detector. Analytes which permeate the membrane were ionized by chemical ionization. The concentrations of the liquid phase products acetic acid, acetoin, 2,3-butanediol and ethanol were monitored as a function of time and quantified relative to external standard solutions. Off-line gas chromatography was utilized to test the accuracy of these measurements and excellent agreement was found. Products of fermentation by the organism Clostridium acetobutylicum have also been examined.

Original languageEnglish
Pages (from-to)105-116
Number of pages12
JournalProcess Control and Quality
Volume1
Issue number2
Publication statusPublished - 1991 Feb 1

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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