Bioremediation of selenium-contaminated soil using earthworm Eisenia fetida: Effects of gut bacteria in feces on the soil microbiome

Yikun Wang, Zi Jing Wang, Jung Chen Huang, Azharuddin Chachar, Chuanqi Zhou, Shengbing He

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Selenium (Se) contamination in the soil poses a food safety risk to humans. The present study was to investigate the role of earthworm Eisenia fetida in soil Se remediation. When exposed to selenite at 4 mg Se/kg, E. fetida efficiently concentrated Se in tissues (24.53 mg Se/kg dry weight), however, only accounting for a minor portion of the added Se. Microbial analysis shows 12 out of 15 functional genera became more abundant in the worm-inhabited soil when exposed to Se, suggesting E. fetida contributed to Se remediation mainly by introducing Se-reducing bacteria to the soil via feces, which were dominated by the genera Pseudomonas (∼62.65%) and Aeromonas (∼29.99%), whose abundance was also significantly boosted in the worm-inhabited soil. However, when isolated from worm feces at 200 mg Se/L, Pseudomonas strains only displayed a high tolerance to Se rather than removal capacity. In contrast, among 4 isolated Aeromonas strains, A. caviae rapidly removing 85.74% of the added selenite, mainly through accumulation (67.38%), while A. hydrophila and A. veronii were more effective at volatilizing Se (27.77% and 24.54%, respectively), and A. media performed best by reducing Se by ∼49.00% under anaerobic conditions. Overall, our findings have highlighted the importance of E. fetida as a key contributor of functional bacteria to the soil microbiome, building a strong foundation for the development of an earthworm-soil system for Se bioremediation.

Original languageEnglish
Article number134544
JournalChemosphere
Volume300
DOIs
Publication statusPublished - 2022 Aug

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • General Chemistry
  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Fingerprint

Dive into the research topics of 'Bioremediation of selenium-contaminated soil using earthworm Eisenia fetida: Effects of gut bacteria in feces on the soil microbiome'. Together they form a unique fingerprint.

Cite this