Black silicon SERS substrate: Effect of surface morphology on SERS detection and application of single algal cell analysis

Yu Luen Deng, Yi Je Juang

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

In this study, we have investigated the effect of the surface morphology of the black silicon substrate on surface enhanced Raman spectroscopy (SERS) and explored its application of single algal cell detection. By adjusting the O2 and SF6 flow rates in the cryogenic plasma etching process, different surface morphologies of the black silicon substrate was produced without performing the lithographic process. It was found the Raman signals were better enhanced as the tip density of the black silicon substrate increased. In addition, as the thickness of the deposited gold layer increased, the SERS effect increased as well, which could be owing to the generation of more hot spots by bridging individual silicon tips through deposition of gold layer. For the black silicon substrate with tip density of 30tips/μm2 and covered by 400nm deposited gold layer, the detection limit of 10fM R6G solution concentration with uniform SERS effect across the substrate was achieved. Furthermore, detection of individual algal cell (Chlorella vulgaris) was performed at the SERS substrate as fabricated and the Raman signals of carotenoid and lipid were substantially enhanced.

Original languageEnglish
Pages (from-to)37-42
Number of pages6
JournalBiosensors and Bioelectronics
Volume53
DOIs
Publication statusPublished - 2014 Mar 15

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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