Bone tissue engineering evaluation based on rat calvaria stromal cells cultured on modified PLGA scaffolds

Yu Chun Wu, Shyh-Yu Shaw, Hong Ru Lin, Tzer-Min Lee, Chyun-Yu Yang

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Using natural materials to coat the scaffolds used for tissue-engineered bone-repair techniques is expected to increase osteoblast adhesion to the scaffold and to express normal physiological function. To test this hypothesis, we therefore modified poly(dl-lactic-co-glycolic acid) (PLGA) substrate by coating it with natural biomaterial solutions of collagen, chitosan, or N-succinyl-chitosan, and then used these three combinations as scaffolds to evaluate their effects on osteoblast attachment, proliferation, and differentiation. The results demonstrated that the pore size of scaffolds ranging from 125-500 μm did not affect the osteoblast phenotype; however, the surface modification of the scaffolds coated with these natural biomaterials did. Collagen increased cell attachment and proliferation, but chitosan and N-succinyl-chitosan decreased them. Chitosan and N-succinyl-chitosan increased differentiation, but collagen decreased it. These results provide us a new strategy for modifying microenvironments to increase osteoblast adhesion, proliferation, and differentiation on PLGA scaffolds, a strategy that might be useful for tissue regeneration.

Original languageEnglish
Pages (from-to)896-904
Number of pages9
JournalBiomaterials
Volume27
Issue number6
DOIs
Publication statusPublished - 2006 Feb 1

Fingerprint

glycolic acid
Tissue Engineering
Stromal Cells
Osteoblasts
Chitosan
Tissue engineering
Skull
Scaffolds
Rats
Bone
Bone and Bones
Collagen
Acids
Biocompatible Materials
Tissue Scaffolds
Biomaterials
Adhesion
Tissue regeneration
Regeneration
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

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Bone tissue engineering evaluation based on rat calvaria stromal cells cultured on modified PLGA scaffolds. / Wu, Yu Chun; Shaw, Shyh-Yu; Lin, Hong Ru; Lee, Tzer-Min; Yang, Chyun-Yu.

In: Biomaterials, Vol. 27, No. 6, 01.02.2006, p. 896-904.

Research output: Contribution to journalArticle

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