C-and D-class MADS-box genes from phalaenopsis equestris (orchidaceae) display functions in gynostemium and ovule development

You Yi Chen, Pei Fang Lee, Yu Yun Hsiao, Wan Lin Wu, Zhao Jun Pan, Yung I. Lee, Ke Wei Liu, Li Jun Chen, Zhong Jian Liu, Wen Chieh Tsai

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

Gynostemium and ovule development in orchid are unique developmental processes in the plant kingdom. Characterization of C-and D-class MADS-box genes could help reveal the molecular mechanisms underlying gynostemium and ovule development in orchids. In this study, we isolated and characterized a C-and a D-class gene, PeMADS1 and PeMADS7, respectively, from Phalaenopsis equestris. These two genes showed parallel spatial and temporal expression profiles, which suggests their cooperation in gynostemium and ovule development. Furthermore, only PeMADS1 was ectopically expressed in the petals of the gylp (gynostemium-like petal) mutant, whose petals were transformed into gynostemium-like structures. Proteinprotein interaction analyses revealed that neither PeMADS1 and PeMADS7 could form a homodimer or a heterodimer. An E-class protein was needed to bridge the interaction between these two proteins. A complementation test revealed that PeMADS1 could rescue the phenotype of the AG mutant. Overexpression of PeMADS7 in Arabidopsis caused typical phenotypes of the D-class gene family. Together, these results indicated that both C-class PeMADS1 and D-class PeMADS7 play important roles in orchid gynostemium and ovule development.

Original languageEnglish
Pages (from-to)1053-1067
Number of pages15
JournalPlant and Cell Physiology
Volume53
Issue number6
DOIs
Publication statusPublished - 2012 Jun

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

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