Cadmium biosorption by polyvinyl alcohol immobilized recombinant Escherichia coli

Wei Chen Kao, Jane Yii Wu, Chia Che Chang, Jo-Shu Chang

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Recombinant Escherichia coli expressing human metallothionein protein was immobilized with polyvinyl alcohol (PVA) for the removal of cadmium from solution. The adsorption ability was strongly affected by pH with optimal performance at pH 5.0, while it was less sensitive to temperature over the range of 20-42 °C. The adsorption kinetics and equilibrium of PVA-immobilized cells was best described by pseudo-second order model and Langmuir isotherm, respectively. Over the Cd concentrations range of 10-150 mg/l, PVA-cells had the highest Cd removal percentage (82.7%) at 10 mg Cd/l and a biomass loading of 15.4 wt.%. Better adsorption ability was obtained when biomass loading was increased, as the highest adsorption capacity of 4.29 mg/g was achieved at 33.0 wt.% of biomass (initial Cd concentration = 100 mg/l). An aqueous solution of 0.01 M Na3NTA displayed the best desorption efficiency (57-89%) for four A/D cycles, while 51-61% of the original adsorption capacity was retained after regeneration.

Original languageEnglish
Pages (from-to)651-658
Number of pages8
JournalJournal of Hazardous Materials
Volume169
Issue number1-3
DOIs
Publication statusPublished - 2009 Sep 30

Fingerprint

Polyvinyl Alcohol
Biosorption
Polyvinyl alcohols
Cadmium
Escherichia coli
Adsorption
alcohol
cadmium
adsorption
Biomass
biomass
Immobilized Proteins
Immobilized Cells
metallothionein
Metallothionein
Isotherms
Regeneration
Desorption
desorption
isotherm

All Science Journal Classification (ASJC) codes

  • Health, Toxicology and Mutagenesis
  • Pollution
  • Waste Management and Disposal
  • Environmental Chemistry
  • Environmental Engineering

Cite this

Kao, Wei Chen ; Wu, Jane Yii ; Chang, Chia Che ; Chang, Jo-Shu. / Cadmium biosorption by polyvinyl alcohol immobilized recombinant Escherichia coli. In: Journal of Hazardous Materials. 2009 ; Vol. 169, No. 1-3. pp. 651-658.
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Cadmium biosorption by polyvinyl alcohol immobilized recombinant Escherichia coli. / Kao, Wei Chen; Wu, Jane Yii; Chang, Chia Che; Chang, Jo-Shu.

In: Journal of Hazardous Materials, Vol. 169, No. 1-3, 30.09.2009, p. 651-658.

Research output: Contribution to journalArticle

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