Calcium dynamics during trap closure visualized in transgenic Venus flytrap

The leaves of the carnivorous plant Venus flytrap, Dionaea muscipula (Dionaea) close rapidly to capture insect prey. The closure response usually requires two successive mechanical stimuli to sensory hairs on the leaf blade within approximately 30 s (refs. ^1–4). An unknown biological system in Dionaea is thought to memorize the first stimulus and transduce the signal from the sensory hair to the leaf blade². Here, we link signal memory to calcium dynamics using transgenic Dionaea expressing a Ca²⁺ sensor. Stimulation of a sensory hair caused an increase in cytosolic Ca²⁺ concentration ([Ca²⁺]_cyt) starting in the sensory hair and spreading to the leaf blade. A second stimulus increased [Ca²⁺]_cyt to an even higher level, meeting a threshold that is correlated to the leaf blade closure. Because [Ca²⁺]_cyt gradually decreased after the first stimulus, the [Ca²⁺]_cyt increase induced by the second stimulus was insufficient to meet the putative threshold for movement after about 30 s. The Ca²⁺ wave triggered by mechanical stimulation moved an order of magnitude faster than that induced by wounding in petioles of Arabidopsis thaliana⁵ and Dionaea. The capacity for rapid movement has evolved repeatedly in flowering plants. This study opens a path to investigate the role of Ca²⁺ in plant movement mechanisms and their evolution.