TY - JOUR
T1 - Caldimonas taiwanensis sp. nov., a amylase producing bacterium isolated from a hot spring
AU - Chen, Wen Ming
AU - Chang, Jo Shu
AU - Chiu, Ching Hsiang
AU - Chang, Shu Chen
AU - Chen, Wen Chieh
AU - Jiang, Chii Ming
N1 - Funding Information:
W.-M. Chen was supported by a grant from the National Science Council, Taipei, Taiwan, Republic of China (NSC 93-2320-B-022-001 and -003).
PY - 2005/7/20
Y1 - 2005/7/20
N2 - During screening for amylase-producing bacteria, a strain designated On1T was isolated from a hot spring located in Pingtung area, which is near the very southern part of Taiwan. Cells of this organism were Gram-negative rods motile by means of a single polar flagellum. Optimum conditions for growth were 55°C and pH 7. Strain On1T grew well in minimal medium containing starch as the sole carbon source, and its extracellular products expressed amylase activity. The 16S rRNA gene sequence analysis indicates that strain On1T is a member of β-Proteobacteria. On the basis of a phylogenetic analysis of 16S rDNA sequences, DNA-DNA similarity data, physiological and biochemical characteristics, as well as fatty acid compositions, the organism belonged to the genus Caldimonas and represented a novel species within this genus. The predominant cellular fatty acids of strain On1T were 16:0 (about 30%), 18:1 ω7c (about 20%) and summed feature 3 (16:1ω7c or 15:0 iso 2OH or both [about 31%]). Its DNA base ratio was 65.9 mol% G+C. We propose to classify strain On1T (=BCRC 17405T=LMG 22827T) as Caldimonas taiwanensis sp. nov.
AB - During screening for amylase-producing bacteria, a strain designated On1T was isolated from a hot spring located in Pingtung area, which is near the very southern part of Taiwan. Cells of this organism were Gram-negative rods motile by means of a single polar flagellum. Optimum conditions for growth were 55°C and pH 7. Strain On1T grew well in minimal medium containing starch as the sole carbon source, and its extracellular products expressed amylase activity. The 16S rRNA gene sequence analysis indicates that strain On1T is a member of β-Proteobacteria. On the basis of a phylogenetic analysis of 16S rDNA sequences, DNA-DNA similarity data, physiological and biochemical characteristics, as well as fatty acid compositions, the organism belonged to the genus Caldimonas and represented a novel species within this genus. The predominant cellular fatty acids of strain On1T were 16:0 (about 30%), 18:1 ω7c (about 20%) and summed feature 3 (16:1ω7c or 15:0 iso 2OH or both [about 31%]). Its DNA base ratio was 65.9 mol% G+C. We propose to classify strain On1T (=BCRC 17405T=LMG 22827T) as Caldimonas taiwanensis sp. nov.
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U2 - 10.1016/j.syapm.2005.02.008
DO - 10.1016/j.syapm.2005.02.008
M3 - Article
C2 - 16094868
AN - SCOPUS:20444395830
SN - 0723-2020
VL - 28
SP - 415
EP - 420
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
IS - 5
ER -