Cardiovascular effects of nitric oxide and adenosine in the nucleus tractus solitarii of rats

Wan Chen Lo, Chung Ren Jan, Sheng-Nan Wu, Ching Jiunn Tseng

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

It has been shown that nitric oxide (NO) is synthesized in the central nervous system as well as in vascular endothelial cells. We recently reported that NO was involved in central cardiovascular regulation, modulated the baroreflex, and was involved in a reciprocal release with excitatory amino acids in the nucleus tractus solitarii (NTS) of rats. We also reported previously that adenosine increased the release of glutamate in the NTS. The purpose of the present study was to investigate the possible interaction of NO and adenosine in the NTS. Male Sprague-Dawley rats were anesthetized with urethane, and blood pressure was monitored intra-arterially. Unilateral microinjection of L-arginine (3.3 nmol/60 nL) into the NTS produced decreases in blood pressure and heart rate. Microinjection of adenosine (2.3 nmol/60 nL) also produced depressive and bradycardic effects. These cardiovascular effects were attenuated by prior administration of the specific adenosine receptor antagonist DPSPX (0.92 nmol). Similarly, prior administration of NO synthase inhibitor N(G)-monomethyl-L-arginine or N(G)-nitro-L-arginine methyl ester significantly attenuated the depressive and bradycardic effects of adenosine. These results demonstrate a reciprocal attenuation of adenosine receptor antagonist and NO synthase inhibitor on L-arginine and adenosine responses, respectively, in the NTS and implicate an interaction between NO and adenosine in central cardiovascular regulation.

Original languageEnglish
Pages (from-to)1034-1038
Number of pages5
JournalHypertension
Volume32
Issue number6
DOIs
Publication statusPublished - 1998 Jan 1

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Solitary Nucleus
Adenosine
Nitric Oxide
Purinergic P1 Receptor Antagonists
Arginine
Microinjections
Nitric Oxide Synthase
Blood Pressure
Excitatory Amino Acids
Baroreflex
Urethane
NG-Nitroarginine Methyl Ester
Sprague Dawley Rats
Glutamic Acid
Central Nervous System
Endothelial Cells
Heart Rate

All Science Journal Classification (ASJC) codes

  • Internal Medicine

Cite this

Lo, Wan Chen ; Jan, Chung Ren ; Wu, Sheng-Nan ; Tseng, Ching Jiunn. / Cardiovascular effects of nitric oxide and adenosine in the nucleus tractus solitarii of rats. In: Hypertension. 1998 ; Vol. 32, No. 6. pp. 1034-1038.
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Cardiovascular effects of nitric oxide and adenosine in the nucleus tractus solitarii of rats. / Lo, Wan Chen; Jan, Chung Ren; Wu, Sheng-Nan; Tseng, Ching Jiunn.

In: Hypertension, Vol. 32, No. 6, 01.01.1998, p. 1034-1038.

Research output: Contribution to journalArticle

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