Caspase-3 enhances lung metastasis and cell migration in a protease-independent mechanism through the ERK pathway

Yu Jung Cheng, Chien Hsin Lee, Yu Ping Lin, Jyun Yuan Huang, Chung Chen Su, Wen Tsan Chang, Bei Chang Yang

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Caspase-3 is known as a cysteine protease that primarily executes the cell death program. However, some tumors express higher levels of caspase-3 in positive correlation with malignancy. Here, we showed that caspase-3 can promote tumor metastasis in a protease-independent mechanism. Ectopic expression of caspase-3 enhanced lung metastasis and cell motility of caspase-3 deficient MCF-7 cells. By contrast, caspase-3 siRNA reduced the invasiveness and metastasis ability of A549 cells that express high level of caspase-3. Moreover, caspase-3 induced ERK activation. Alteration of caspase-3 by introducing non-processable mutation at its cleavage site or treatment of caspase-3 inhibitor did not diminish the caspase-3-associated increases in ERK phosphorylation and cell migration. Confocal microscopy study showed that caspase-3 was not physically associated with ERK. Inhibiting ceramide formation by blockage of the ceramide synthase or acid sphingomyelinase activity resulted in significant reduction of ERK phosphorylation and cell migration. In summary, caspase-3 induces ERK activation through a ceramide-dependant, protease activity-independent mechanism, which represents a novel role of caspase-3 in tumor metastasis.

Original languageEnglish
Pages (from-to)1278-1285
Number of pages8
JournalInternational Journal of Cancer
Volume123
Issue number6
DOIs
Publication statusPublished - 2008 Sep 15

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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