Caspase-8 acts as a key upstream executor of mitochondria during justicidin A-induced apoptosis in human hepatoma cells

Chun Li Su, Lynn L.H. Huang, Li Min Huang, Jenq Chang Lee, Chun Nan Lin, Shen Jeu Won

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Justicia procumbens is a traditional Taiwanese herbal remedy used to treat fever, pain, and cancer. Justicidin A, isolated from Justicia procumbens, has been reported to suppress in vitro growth of several tumor cell lines as well as hepatoma cells. In this study, justicidin A activated caspase-8 to increase tBid, disrupted mitochondrial membrane potential (Δψm), and caused the release of cytochrome c and Smac/DIABLO in Hep 3B and Hep G2 cells. Justicidin A also reduced Bcl-xL and increased Bax and Bak in mitochondria. Caspase-8 inhibitor (Z-IETD) attenuated the justicidin A-induced disruption of Δψm. Growth of Hep 3B implanted in NOD-SCID mice was suppressed significantly by oral justicidin A (20 mg/kg/day). These results indicate that justicidin A-induced apoptosis in these cells proceeds via caspase-8 and is followed by mitochondrial disruption. Supplementary materials are available at http://myweb.ncku.edu.tw/~a725/.

Original languageEnglish
Pages (from-to)3185-3191
Number of pages7
JournalFEBS Letters
Volume580
Issue number13
DOIs
Publication statusPublished - 2006 May 29

Fingerprint

Mitochondria
Caspase 8
Hepatocellular Carcinoma
Apoptosis
Justicia
Inbred NOD Mouse
Caspase Inhibitors
SCID Mice
Mitochondrial Membrane Potential
Hep G2 Cells
Growth
Cytochromes c
Tumor Cell Line
justicidin A
Tumors
Fever
Cells
Membranes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

@article{55a49a99263948eca00223bb16560a5e,
title = "Caspase-8 acts as a key upstream executor of mitochondria during justicidin A-induced apoptosis in human hepatoma cells",
abstract = "Justicia procumbens is a traditional Taiwanese herbal remedy used to treat fever, pain, and cancer. Justicidin A, isolated from Justicia procumbens, has been reported to suppress in vitro growth of several tumor cell lines as well as hepatoma cells. In this study, justicidin A activated caspase-8 to increase tBid, disrupted mitochondrial membrane potential (Δψm), and caused the release of cytochrome c and Smac/DIABLO in Hep 3B and Hep G2 cells. Justicidin A also reduced Bcl-xL and increased Bax and Bak in mitochondria. Caspase-8 inhibitor (Z-IETD) attenuated the justicidin A-induced disruption of Δψm. Growth of Hep 3B implanted in NOD-SCID mice was suppressed significantly by oral justicidin A (20 mg/kg/day). These results indicate that justicidin A-induced apoptosis in these cells proceeds via caspase-8 and is followed by mitochondrial disruption. Supplementary materials are available at http://myweb.ncku.edu.tw/~a725/.",
author = "Su, {Chun Li} and Huang, {Lynn L.H.} and Huang, {Li Min} and Lee, {Jenq Chang} and Lin, {Chun Nan} and Won, {Shen Jeu}",
year = "2006",
month = "5",
day = "29",
doi = "10.1016/j.febslet.2006.04.085",
language = "English",
volume = "580",
pages = "3185--3191",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "13",

}

Caspase-8 acts as a key upstream executor of mitochondria during justicidin A-induced apoptosis in human hepatoma cells. / Su, Chun Li; Huang, Lynn L.H.; Huang, Li Min; Lee, Jenq Chang; Lin, Chun Nan; Won, Shen Jeu.

In: FEBS Letters, Vol. 580, No. 13, 29.05.2006, p. 3185-3191.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Caspase-8 acts as a key upstream executor of mitochondria during justicidin A-induced apoptosis in human hepatoma cells

AU - Su, Chun Li

AU - Huang, Lynn L.H.

AU - Huang, Li Min

AU - Lee, Jenq Chang

AU - Lin, Chun Nan

AU - Won, Shen Jeu

PY - 2006/5/29

Y1 - 2006/5/29

N2 - Justicia procumbens is a traditional Taiwanese herbal remedy used to treat fever, pain, and cancer. Justicidin A, isolated from Justicia procumbens, has been reported to suppress in vitro growth of several tumor cell lines as well as hepatoma cells. In this study, justicidin A activated caspase-8 to increase tBid, disrupted mitochondrial membrane potential (Δψm), and caused the release of cytochrome c and Smac/DIABLO in Hep 3B and Hep G2 cells. Justicidin A also reduced Bcl-xL and increased Bax and Bak in mitochondria. Caspase-8 inhibitor (Z-IETD) attenuated the justicidin A-induced disruption of Δψm. Growth of Hep 3B implanted in NOD-SCID mice was suppressed significantly by oral justicidin A (20 mg/kg/day). These results indicate that justicidin A-induced apoptosis in these cells proceeds via caspase-8 and is followed by mitochondrial disruption. Supplementary materials are available at http://myweb.ncku.edu.tw/~a725/.

AB - Justicia procumbens is a traditional Taiwanese herbal remedy used to treat fever, pain, and cancer. Justicidin A, isolated from Justicia procumbens, has been reported to suppress in vitro growth of several tumor cell lines as well as hepatoma cells. In this study, justicidin A activated caspase-8 to increase tBid, disrupted mitochondrial membrane potential (Δψm), and caused the release of cytochrome c and Smac/DIABLO in Hep 3B and Hep G2 cells. Justicidin A also reduced Bcl-xL and increased Bax and Bak in mitochondria. Caspase-8 inhibitor (Z-IETD) attenuated the justicidin A-induced disruption of Δψm. Growth of Hep 3B implanted in NOD-SCID mice was suppressed significantly by oral justicidin A (20 mg/kg/day). These results indicate that justicidin A-induced apoptosis in these cells proceeds via caspase-8 and is followed by mitochondrial disruption. Supplementary materials are available at http://myweb.ncku.edu.tw/~a725/.

UR - http://www.scopus.com/inward/record.url?scp=33646595001&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646595001&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2006.04.085

DO - 10.1016/j.febslet.2006.04.085

M3 - Article

C2 - 16684533

AN - SCOPUS:33646595001

VL - 580

SP - 3185

EP - 3191

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 13

ER -