Cell adhesion and related phenomena on the surface-modified Au-deposited nerve microelectrode examined by total impedance measurement and cell detachment tests

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This study investigated alkanethiolate self-assembled monolayers (SAMs) of varied chain lengths adsorbed upon novel Au-coated microelectrodes, of which the surface properties were quantitatively evaluated by surface characterization and 3T3 fibroblast cell adhesion, total impedance and cell detachment tests. Thin-film SAMs adsorbed upon Au/PI/Si provided a hydrophobic or passive surface with increased water contact angle and initial total impedance. From cell adhesion tests, we can observe that the film formed as a dense-packed spacer resulted in incomplete cell sealing of 3T3 cells upon the surface-modified microelectrode. Thus the decrease in cell coverage rate and in the slope in association with total impedance as a function of cell-surface reaction time can be found. To study the adhesion force of a comparable single cell attached upon varied modified surfaces, a cell detachment test using a triangular probe tip of a well defined cantilever was carried out in medium containing fibroblast cells. Overall, both the peak force and the work required to detach a comparable single cell from the anchoring domain corresponded well to the increased length of alkyl chains adsorbed upon Au/PI/Si. Both measurements on the SAM modified surfaces demonstrated much smaller values than those on the pristine Au/PI/Si surface. These results concluded that a cell-repulsive characteristic was clearly formed on the SAM modified microelectrode surface. The non-adhering properties of surface-modified microelectrodes should provide better sensitivity for neuromuscular stimulation as well as for the recording of infinitesimal neural signals in future applications of neural prostheses.

Original languageEnglish
Pages (from-to)2449-2457
Number of pages9
JournalNanotechnology
Volume17
Issue number10
DOIs
Publication statusPublished - 2006 May 28

Fingerprint

Microelectrodes
Cell adhesion
impedance measurement
nerves
detachment
adhesion
Self assembled monolayers
cells
Fibroblasts
fibroblasts
impedance
Neural prostheses
Surface reactions
adhesion tests
Chain length
Contact angle
Surface properties
sealing
stimulation
Adhesion

All Science Journal Classification (ASJC) codes

  • Engineering (miscellaneous)
  • Materials Science(all)
  • Physics and Astronomy (miscellaneous)

Cite this

@article{fdeb5b962c6d4db8bcbd9ef004584905,
title = "Cell adhesion and related phenomena on the surface-modified Au-deposited nerve microelectrode examined by total impedance measurement and cell detachment tests",
abstract = "This study investigated alkanethiolate self-assembled monolayers (SAMs) of varied chain lengths adsorbed upon novel Au-coated microelectrodes, of which the surface properties were quantitatively evaluated by surface characterization and 3T3 fibroblast cell adhesion, total impedance and cell detachment tests. Thin-film SAMs adsorbed upon Au/PI/Si provided a hydrophobic or passive surface with increased water contact angle and initial total impedance. From cell adhesion tests, we can observe that the film formed as a dense-packed spacer resulted in incomplete cell sealing of 3T3 cells upon the surface-modified microelectrode. Thus the decrease in cell coverage rate and in the slope in association with total impedance as a function of cell-surface reaction time can be found. To study the adhesion force of a comparable single cell attached upon varied modified surfaces, a cell detachment test using a triangular probe tip of a well defined cantilever was carried out in medium containing fibroblast cells. Overall, both the peak force and the work required to detach a comparable single cell from the anchoring domain corresponded well to the increased length of alkyl chains adsorbed upon Au/PI/Si. Both measurements on the SAM modified surfaces demonstrated much smaller values than those on the pristine Au/PI/Si surface. These results concluded that a cell-repulsive characteristic was clearly formed on the SAM modified microelectrode surface. The non-adhering properties of surface-modified microelectrodes should provide better sensitivity for neuromuscular stimulation as well as for the recording of infinitesimal neural signals in future applications of neural prostheses.",
author = "Chang, {Cheng Hung} and Jiunn-Der Liao and Jia-Jin Chen and Ming-Shaung Ju and Chou-Ching Lin",
year = "2006",
month = "5",
day = "28",
doi = "10.1088/0957-4484/17/10/004",
language = "English",
volume = "17",
pages = "2449--2457",
journal = "Nanotechnology",
issn = "0957-4484",
publisher = "IOP Publishing Ltd.",
number = "10",

}

TY - JOUR

T1 - Cell adhesion and related phenomena on the surface-modified Au-deposited nerve microelectrode examined by total impedance measurement and cell detachment tests

AU - Chang, Cheng Hung

AU - Liao, Jiunn-Der

AU - Chen, Jia-Jin

AU - Ju, Ming-Shaung

AU - Lin, Chou-Ching

PY - 2006/5/28

Y1 - 2006/5/28

N2 - This study investigated alkanethiolate self-assembled monolayers (SAMs) of varied chain lengths adsorbed upon novel Au-coated microelectrodes, of which the surface properties were quantitatively evaluated by surface characterization and 3T3 fibroblast cell adhesion, total impedance and cell detachment tests. Thin-film SAMs adsorbed upon Au/PI/Si provided a hydrophobic or passive surface with increased water contact angle and initial total impedance. From cell adhesion tests, we can observe that the film formed as a dense-packed spacer resulted in incomplete cell sealing of 3T3 cells upon the surface-modified microelectrode. Thus the decrease in cell coverage rate and in the slope in association with total impedance as a function of cell-surface reaction time can be found. To study the adhesion force of a comparable single cell attached upon varied modified surfaces, a cell detachment test using a triangular probe tip of a well defined cantilever was carried out in medium containing fibroblast cells. Overall, both the peak force and the work required to detach a comparable single cell from the anchoring domain corresponded well to the increased length of alkyl chains adsorbed upon Au/PI/Si. Both measurements on the SAM modified surfaces demonstrated much smaller values than those on the pristine Au/PI/Si surface. These results concluded that a cell-repulsive characteristic was clearly formed on the SAM modified microelectrode surface. The non-adhering properties of surface-modified microelectrodes should provide better sensitivity for neuromuscular stimulation as well as for the recording of infinitesimal neural signals in future applications of neural prostheses.

AB - This study investigated alkanethiolate self-assembled monolayers (SAMs) of varied chain lengths adsorbed upon novel Au-coated microelectrodes, of which the surface properties were quantitatively evaluated by surface characterization and 3T3 fibroblast cell adhesion, total impedance and cell detachment tests. Thin-film SAMs adsorbed upon Au/PI/Si provided a hydrophobic or passive surface with increased water contact angle and initial total impedance. From cell adhesion tests, we can observe that the film formed as a dense-packed spacer resulted in incomplete cell sealing of 3T3 cells upon the surface-modified microelectrode. Thus the decrease in cell coverage rate and in the slope in association with total impedance as a function of cell-surface reaction time can be found. To study the adhesion force of a comparable single cell attached upon varied modified surfaces, a cell detachment test using a triangular probe tip of a well defined cantilever was carried out in medium containing fibroblast cells. Overall, both the peak force and the work required to detach a comparable single cell from the anchoring domain corresponded well to the increased length of alkyl chains adsorbed upon Au/PI/Si. Both measurements on the SAM modified surfaces demonstrated much smaller values than those on the pristine Au/PI/Si surface. These results concluded that a cell-repulsive characteristic was clearly formed on the SAM modified microelectrode surface. The non-adhering properties of surface-modified microelectrodes should provide better sensitivity for neuromuscular stimulation as well as for the recording of infinitesimal neural signals in future applications of neural prostheses.

UR - http://www.scopus.com/inward/record.url?scp=33646716485&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646716485&partnerID=8YFLogxK

U2 - 10.1088/0957-4484/17/10/004

DO - 10.1088/0957-4484/17/10/004

M3 - Article

C2 - 21727489

AN - SCOPUS:33646716485

VL - 17

SP - 2449

EP - 2457

JO - Nanotechnology

JF - Nanotechnology

SN - 0957-4484

IS - 10

ER -