Cell line studies of glioblastoma and malignant glioma using a newly discovered prenylated chalcone

Chih Chuan Teng, Chun I. Sze, Ting Chung Wang, Ching Yi Lien, Wayne C. Liao

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Context: A newly discovered geranyl prenylated chalcone, semisynthesized from naturally occurring nymphaeol C, has the ability to inhibit the growth of CNS1 (glioblastoma) and 13-06 (malignant glioma) cells. A second-order regression model was established to predict the normalized cell viability of CNS1 and 13-06 cells. Objective: The goal of this study is to evaluate the influence of prenylated chalcone on the glioblastoma and malignant glioma cell lines. For the first time, response surface methodology (RSM) has been introduced to perform a cell line study. Materials and methods: A newly discovered prenylated chalcone was used. This compound is a member of the flavonoid family and possesses a common phenylbenzopyrone structure. Two independent factors, including prenylated chalcone concentration and uptake time, were carefully evaluated by a 2 2 factorial design. RSM was introduced as a new method for CNS1 and 13-06 cell line studies. Results: For CNS1 cells, the least inhibition uptake time was 20.7h, and the least inhibition dose was 12.4 μg/ml. For 13-06 cells, the best inhibition uptake time was 26.2h, and the least inhibition dose was 12.0 μg/ml. Discussion and conclusion: The RSM model successfully predicted the normalized cell viability of CNS1 and 13-06 cells through the use of prenylated chalcone. The results obtained in this study will be useful for further studies on the use of prenylated chalcone.

Original languageEnglish
Pages (from-to)314-318
Number of pages5
JournalPharmaceutical Biology
Volume49
Issue number3
DOIs
Publication statusPublished - 2011 Mar

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

Fingerprint

Dive into the research topics of 'Cell line studies of glioblastoma and malignant glioma using a newly discovered prenylated chalcone'. Together they form a unique fingerprint.

Cite this