Cellular Composition Changes and Nitrogen Uptake under Extra-Limited Nitrogen Conditions by Thermosynechococcus sp. CL-1 Carbon Biofixation

Tseng Chi-Ming, Ko Tzu-Hsing, Hsueh Hsin-Ta, Chen Hsing-Hui, Ray Dah-Tong, Shen Yun-Hwei, Chu Hsin

Research output: Contribution to journalArticle

Abstract

Two types of culture systems were used (continuous and batch) which were fed using a simulated absorbent from a scrubber with carbonate/bicarbonate as the carbon source and nitrate as the nitrogen source by a thermophile strain, Thermosynechococcus sp. CL-1 (TCL-1) at 50°C. The lipid, carbohydrate, and protein cellular components which can be used as bioenergy precursors along with their content as a function of various C/N ratios are quantified. Maximum lipid productivity of about 150 mg L-1 d-1 is obtained while the CO2 uptake rate is 917 mg L-1 d-1 at a dilution rate of 0.06 h-1 when both carbon and nitrogen sources are not limited. With high range of nitrogen concentrations batch culture test, TCL-1 reveals extra-high affinity on nitrogen source under limited carbon source conditions since the affinity constant is 0.12 mM. In addition, the flow of carbon fixed during photosynthesis seems to switch from the protein synthesis pathway to forming carbohydrate rather than lipid under N-limitation and a high C/N ratio for TCL-1, resulting in a maximal carbohydrate content of 61%. Consequently, TCL-1 is an appropriate candidate to treat the wastewater of environment and produce the bioenergy precursors under extreme limited nitrogen conditions.

Original languageEnglish
Article number5247265
JournalJournal of Chemistry
Volume2016
DOIs
Publication statusPublished - 2016 Jan 1

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Nitrogen
Carbon
Chemical analysis
Carbohydrates
Lipids
Scrubbers
Photosynthesis
Carbonates
Bicarbonates
Nitrates
Dilution
Proteins
Wastewater
Productivity
Switches

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

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title = "Cellular Composition Changes and Nitrogen Uptake under Extra-Limited Nitrogen Conditions by Thermosynechococcus sp. CL-1 Carbon Biofixation",
abstract = "Two types of culture systems were used (continuous and batch) which were fed using a simulated absorbent from a scrubber with carbonate/bicarbonate as the carbon source and nitrate as the nitrogen source by a thermophile strain, Thermosynechococcus sp. CL-1 (TCL-1) at 50°C. The lipid, carbohydrate, and protein cellular components which can be used as bioenergy precursors along with their content as a function of various C/N ratios are quantified. Maximum lipid productivity of about 150 mg L-1 d-1 is obtained while the CO2 uptake rate is 917 mg L-1 d-1 at a dilution rate of 0.06 h-1 when both carbon and nitrogen sources are not limited. With high range of nitrogen concentrations batch culture test, TCL-1 reveals extra-high affinity on nitrogen source under limited carbon source conditions since the affinity constant is 0.12 mM. In addition, the flow of carbon fixed during photosynthesis seems to switch from the protein synthesis pathway to forming carbohydrate rather than lipid under N-limitation and a high C/N ratio for TCL-1, resulting in a maximal carbohydrate content of 61{\%}. Consequently, TCL-1 is an appropriate candidate to treat the wastewater of environment and produce the bioenergy precursors under extreme limited nitrogen conditions.",
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Cellular Composition Changes and Nitrogen Uptake under Extra-Limited Nitrogen Conditions by Thermosynechococcus sp. CL-1 Carbon Biofixation. / Chi-Ming, Tseng; Tzu-Hsing, Ko; Hsin-Ta, Hsueh; Hsing-Hui, Chen; Dah-Tong, Ray; Yun-Hwei, Shen; Hsin, Chu.

In: Journal of Chemistry, Vol. 2016, 5247265, 01.01.2016.

Research output: Contribution to journalArticle

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AU - Chi-Ming, Tseng

AU - Tzu-Hsing, Ko

AU - Hsin-Ta, Hsueh

AU - Hsing-Hui, Chen

AU - Dah-Tong, Ray

AU - Yun-Hwei, Shen

AU - Hsin, Chu

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