Cellular polyamines promote amyloid-Beta (Aβ) peptide fibrillation and modulate the aggregation pathways

Jinghui Luo, Chien Hung Yu, Huixin Yu, Rok Borstnar, Shina C.L. Kamerlin, Astrid Gräslund, Jan Pieter Abrahams, Sebastian K.T.S. Wärmländer

Research output: Contribution to journalArticlepeer-review

62 Citations (Scopus)

Abstract

The cellular polyamines spermine, spermidine, and their metabolic precursor putrescine, have long been associated with cell-growth, tumor-related gene regulations, and Alzheimer's disease. Here, we show by in vitro spectroscopy and AFM imaging, that these molecules promote aggregation of amyloid-beta (Aβ) peptides into fibrils and modulate the aggregation pathways. NMR measurements showed that the three polyamines share a similar binding mode to monomeric Aβ(1-40) peptide. Kinetic ThT studies showed that already very low polyamine concentrations promote amyloid formation: addition of 10 μM spermine (normal intracellular concentration is ∼1 mM) significantly decreased the lag and transition times of the aggregation process. Spermidine and putrescine additions yielded similar but weaker effects. CD measurements demonstrated that the three polyamines induce different aggregation pathways, involving different forms of induced secondary structure. This is supported by AFM images showing that the three polyamines induce Aβ(1-40) aggregates with different morphologies. The results reinforce the notion that designing suitable ligands which modulate the aggregation of Aβ peptides toward minimally toxic pathways may be a possible therapeutic strategy for Alzheimer's disease.

Original languageEnglish
Pages (from-to)454-462
Number of pages9
JournalACS Chemical Neuroscience
Volume4
Issue number3
DOIs
Publication statusPublished - 2013 Mar 20

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Cognitive Neuroscience
  • Cell Biology

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