Cellulosic hydrogen production with a sequencing bacterial hydrolysis and dark fermentation strategy

Yung Chung Lo, Ming Der Bai, Wen Ming Chen, Jo Shu Chang

Research output: Contribution to journalArticlepeer-review

84 Citations (Scopus)


In this study, cellulose hydrolysis activity of two mixed bacterial consortia (NS and QS) was investigated. Combination of NS culture and BHM medium exhibited better hydrolytic activity under the optimal condition of 35 °C, initial pH 7.0, and 100 rpm agitation. The NS culture could hydrolyze carboxymethyl cellulose (CMC), rice husk, bagasse and filter paper, among which CMC gave the best hydrolysis performance. The CMC hydrolysis efficiency increased with increasing CMC concentration from 5 to 50 g/l. With a CMC concentration of 10 g/l, the total reducing sugar (RS) production and the RS producing rate reached 5531.0 mg/l and 92.9 mg/l/h, respectively. Furthermore, seven H2-producing bacterial isolates (mainly Clostridium species) were used to convert the cellulose hydrolysate into H2 energy. With an initial RS concentration of 0.8 g/l, the H2 production and yield was approximately 23.8 ml/l and 1.21 mmol H2/g RS (0.097 mmol H2/g cellulose), respectively.

Original languageEnglish
Pages (from-to)8299-8303
Number of pages5
JournalBioresource technology
Issue number17
Publication statusPublished - 2008 Nov

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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