Characteristics of carbapenemase-producing enterobacteriaceae in wastewater revealed by genomic analysis

Ryota Gomi, Tomonari Matsuda, Masaki Yamamoto, Pei Hsin Chou, Michio Tanaka, Satoshi Ichiyama, Minoru Yoneda, Yasufumi Matsumura

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Wastewater is considered a major source of antibiotic-resistant bacteria released into the environment. Here, we characterized carbapenemase-producing Enterobacteriaceae (CPE) in wastewater by whole-genome analysis. Wastewater samples (n = 40) were collected from municipal wastewater treatment plants and hospital wastewater in Japan and Taiwan. Samples were screened for CPE using selective media, and the obtained isolates were sequenced using an Illumina MiSeq. The isolates (n 45) included the following microorganisms: Klebsiella quasipneumoniae (n = 12), Escherichia coli (n = 10), Enterobacter cloacae complex (n = 10), Klebsiella pneumoniae (n = 8), Klebsiella variicola (n = 2), Raoultella ornithinolytica (n = 1), Citrobacter freundii (n = 1), and Citrobacter amalonaticus (n = 1). Among the 45 isolates, 38 harbored at least one carbapenemase-encoding gene. Of these, the blaGES (blaGES-5, blaGES-6, and blaGES-24) genes were found in 29 isolates. The genes were situated in novel class 1 integrons, but the integron structures were different between the Japanese (In1439 with blaGES-24 and In1440 with blaGES-5) and Taiwanese (In1441 with blaGES-5 and In1442 with blaGES-6) isolates. Other carbapenemase-encoding genes (blaVIM-1, blaNDM-5, blaIMP-8, blaIMP-19, and blaKPC-2) were found in one to three isolates. Notably, class 1 integrons previously reported among clinical isolates obtained in the same regions as the present study, namely, In477 with blaIMP-19 and In73 with blaIMP-8, were found among the Japanese and Taiwanese isolates, respectively. The results indicate that CPE with various carbapenemase-encoding genes in different genetic contexts were present in biologically treated wastewater, highlighting the need to monitor for antibiotic resistance in wastewater.

Original languageEnglish
Article numbere02501-17
JournalAntimicrobial agents and chemotherapy
Volume62
Issue number5
DOIs
Publication statusPublished - 2018 May

Fingerprint

Enterobacteriaceae
Waste Water
Integrons
Klebsiella
Genes
Citrobacter freundii
Citrobacter
Enterobacter cloacae
Klebsiella pneumoniae
Microbial Drug Resistance
carbapenemase
Taiwan
Japan
Genome
Escherichia coli
Anti-Bacterial Agents
Bacteria

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Gomi, Ryota ; Matsuda, Tomonari ; Yamamoto, Masaki ; Chou, Pei Hsin ; Tanaka, Michio ; Ichiyama, Satoshi ; Yoneda, Minoru ; Matsumura, Yasufumi. / Characteristics of carbapenemase-producing enterobacteriaceae in wastewater revealed by genomic analysis. In: Antimicrobial agents and chemotherapy. 2018 ; Vol. 62, No. 5.
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Characteristics of carbapenemase-producing enterobacteriaceae in wastewater revealed by genomic analysis. / Gomi, Ryota; Matsuda, Tomonari; Yamamoto, Masaki; Chou, Pei Hsin; Tanaka, Michio; Ichiyama, Satoshi; Yoneda, Minoru; Matsumura, Yasufumi.

In: Antimicrobial agents and chemotherapy, Vol. 62, No. 5, e02501-17, 05.2018.

Research output: Contribution to journalArticle

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AU - Gomi, Ryota

AU - Matsuda, Tomonari

AU - Yamamoto, Masaki

AU - Chou, Pei Hsin

AU - Tanaka, Michio

AU - Ichiyama, Satoshi

AU - Yoneda, Minoru

AU - Matsumura, Yasufumi

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