Characteristics of five monoclonal anti-alpha-fetoprotein antibodies

R. Wellerson, Shyh-Yu Shaw, P. Kaplan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Five monoclonal anti-alpha-fetoprotein (AFP) antibodies (IgG1) were isolated from six fusions. They were characterized by examining titration values, affinity constants, isotypes, and epitope binding. Employing an epitope-blocking procedure which was based on the exclusion of like antibodies, four of the five monoclonal antibodies were found to be different. Antibody individuality was further demonstrated by comparison of isoelectric focusing patterns. The existence of four distinct anti-AFP antibodies strongly suggests the presence of at least that many epitopes on the AFP molecule.

Original languageEnglish
Pages (from-to)177-185
Number of pages9
JournalHybridoma
Volume3
Issue number2
DOIs
Publication statusPublished - 1984 Jan 1

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alpha-Fetoproteins
Epitopes
Antibodies
Isoelectric Focusing
Individuality
Immunoglobulin G
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Wellerson, R. ; Shaw, Shyh-Yu ; Kaplan, P. / Characteristics of five monoclonal anti-alpha-fetoprotein antibodies. In: Hybridoma. 1984 ; Vol. 3, No. 2. pp. 177-185.
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Characteristics of five monoclonal anti-alpha-fetoprotein antibodies. / Wellerson, R.; Shaw, Shyh-Yu; Kaplan, P.

In: Hybridoma, Vol. 3, No. 2, 01.01.1984, p. 177-185.

Research output: Contribution to journalArticle

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