Characterization and application of Carica papaya lipase to the dynamic kinetic resolution of (R,S)-naproxen thioester

I-Son Ng, Shau Wei Tsai

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

With pH, temperature and metal ions as the parameters, the lipolysis of olive oil in buffers via a partially purified Carica papaya lipase (pCPL) was investigated and compared with that via crude preparation (CPL). The lipase-catalyzed hydrolysis of (R,S)-naproxen thioester in water-saturated organic solvents was then employed as the other model system, in which pCPL was found to be superior to CPL and Candida rugosa lipase (CRL) when the enzyme activity, thermal stability, enantioselectivty and reuse of the recovered lipase was considered. Moreover, in the pCPL-catalyzed dynamic kinetic resolution of(R,S)-naproxen thioester, the concentration of trioctylamine added as a racemization catalyst was found to have profound effects on the kinetic constants for (R)- and (S)-thioester.

Original languageEnglish
Pages (from-to)375-382
Number of pages8
JournalJournal of the Chinese Institute of Chemical Engineers
Volume37
Issue number4
Publication statusPublished - 2006 Jul 1

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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