Abstract
We communicated the construction, characterization and application of microdialysis probes with an active exchange length that is suitable for experiments involving small-size nuclei in rat brain. Using substance P (SP) as the test substance, we determined that probeds with an active exchange length of 180-200 μm exhibited an in vitro recovery of 14.2 ± 0.8% and in vivo recovery at the nucleus tractus solitarii (NTS) of 24.9 ± 1.7% for the undecapeptide, calibrated at an infusion rate of μl/min. We also demonstrated that microinfusion of SP via these probes into the NTS allowed for a correlation of changes in tissue levels of both SP (exogenous substance) and norepinephrine (endogenous substance) with alterations in baroreceptor reflex responses (physiologic phenomenon).
| Original language | English |
|---|---|
| Pages (from-to) | 137-140 |
| Number of pages | 4 |
| Journal | Neuroscience Letters |
| Volume | 175 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 1994 Jul 4 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
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Characterization and application of microdialysis probes with an active exchange length compatible with small-size brain nuclei in the rat. / Tsou, Mei Yung; Len, Wen Bin; Zhang, Ya-Wen; Chan, Julie Y.H.; Lee, Tak Yu; Chan, Samuel H.H.
In: Neuroscience Letters, Vol. 175, No. 1-2, 04.07.1994, p. 137-140.Research output: Contribution to journal › Article
TY - JOUR
T1 - Characterization and application of microdialysis probes with an active exchange length compatible with small-size brain nuclei in the rat
AU - Tsou, Mei Yung
AU - Len, Wen Bin
AU - Zhang, Ya-Wen
AU - Chan, Julie Y.H.
AU - Lee, Tak Yu
AU - Chan, Samuel H.H.
PY - 1994/7/4
Y1 - 1994/7/4
N2 - We communicated the construction, characterization and application of microdialysis probes with an active exchange length that is suitable for experiments involving small-size nuclei in rat brain. Using substance P (SP) as the test substance, we determined that probeds with an active exchange length of 180-200 μm exhibited an in vitro recovery of 14.2 ± 0.8% and in vivo recovery at the nucleus tractus solitarii (NTS) of 24.9 ± 1.7% for the undecapeptide, calibrated at an infusion rate of μl/min. We also demonstrated that microinfusion of SP via these probes into the NTS allowed for a correlation of changes in tissue levels of both SP (exogenous substance) and norepinephrine (endogenous substance) with alterations in baroreceptor reflex responses (physiologic phenomenon).
AB - We communicated the construction, characterization and application of microdialysis probes with an active exchange length that is suitable for experiments involving small-size nuclei in rat brain. Using substance P (SP) as the test substance, we determined that probeds with an active exchange length of 180-200 μm exhibited an in vitro recovery of 14.2 ± 0.8% and in vivo recovery at the nucleus tractus solitarii (NTS) of 24.9 ± 1.7% for the undecapeptide, calibrated at an infusion rate of μl/min. We also demonstrated that microinfusion of SP via these probes into the NTS allowed for a correlation of changes in tissue levels of both SP (exogenous substance) and norepinephrine (endogenous substance) with alterations in baroreceptor reflex responses (physiologic phenomenon).
UR - http://www.scopus.com/inward/record.url?scp=0028340804&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028340804&partnerID=8YFLogxK
U2 - 10.1016/0304-3940(94)91098-7
DO - 10.1016/0304-3940(94)91098-7
M3 - Article
VL - 175
SP - 137
EP - 140
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1-2
ER -