Characterization and application of microdialysis probes with an active exchange length compatible with small-size brain nuclei in the rat

Mei Yung Tsou, Wen Bin Len, Alice Y.W. Chang, Julie Y.H. Chan, Tak Yu Lee, Samuel H.H. Chan

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

We communicated the construction, characterization and application of microdialysis probes with an active exchange length that is suitable for experiments involving small-size nuclei in rat brain. Using substance P (SP) as the test substance, we determined that probeds with an active exchange length of 180-200 μm exhibited an in vitro recovery of 14.2 ± 0.8% and in vivo recovery at the nucleus tractus solitarii (NTS) of 24.9 ± 1.7% for the undecapeptide, calibrated at an infusion rate of μl/min. We also demonstrated that microinfusion of SP via these probes into the NTS allowed for a correlation of changes in tissue levels of both SP (exogenous substance) and norepinephrine (endogenous substance) with alterations in baroreceptor reflex responses (physiologic phenomenon).

Original languageEnglish
Pages (from-to)137-140
Number of pages4
JournalNeuroscience Letters
Volume175
Issue number1-2
DOIs
Publication statusPublished - 1994 Jul 4

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Characterization and application of microdialysis probes with an active exchange length compatible with small-size brain nuclei in the rat'. Together they form a unique fingerprint.

Cite this