Characterization and application of microdialysis probes with an active exchange length compatible with small-size brain nuclei in the rat

Mei Yung Tsou, Wen Bin Len, Ya-Wen Zhang, Julie Y.H. Chan, Tak Yu Lee, Samuel H.H. Chan

Research output: Contribution to journalArticle

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Abstract

We communicated the construction, characterization and application of microdialysis probes with an active exchange length that is suitable for experiments involving small-size nuclei in rat brain. Using substance P (SP) as the test substance, we determined that probeds with an active exchange length of 180-200 μm exhibited an in vitro recovery of 14.2 ± 0.8% and in vivo recovery at the nucleus tractus solitarii (NTS) of 24.9 ± 1.7% for the undecapeptide, calibrated at an infusion rate of μl/min. We also demonstrated that microinfusion of SP via these probes into the NTS allowed for a correlation of changes in tissue levels of both SP (exogenous substance) and norepinephrine (endogenous substance) with alterations in baroreceptor reflex responses (physiologic phenomenon).

Original languageEnglish
Pages (from-to)137-140
Number of pages4
JournalNeuroscience Letters
Volume175
Issue number1-2
DOIs
Publication statusPublished - 1994 Jul 4

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Microdialysis
Substance P
Solitary Nucleus
Brain
Baroreflex
Norepinephrine

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Tsou, Mei Yung ; Len, Wen Bin ; Zhang, Ya-Wen ; Chan, Julie Y.H. ; Lee, Tak Yu ; Chan, Samuel H.H. / Characterization and application of microdialysis probes with an active exchange length compatible with small-size brain nuclei in the rat. In: Neuroscience Letters. 1994 ; Vol. 175, No. 1-2. pp. 137-140.
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Characterization and application of microdialysis probes with an active exchange length compatible with small-size brain nuclei in the rat. / Tsou, Mei Yung; Len, Wen Bin; Zhang, Ya-Wen; Chan, Julie Y.H.; Lee, Tak Yu; Chan, Samuel H.H.

In: Neuroscience Letters, Vol. 175, No. 1-2, 04.07.1994, p. 137-140.

Research output: Contribution to journalArticle

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