Characterization and application of microdialysis probes with an active exchange length compatible with small-size brain nuclei in the rat

Mei Yung Tsou; Wen Bin Len; Alice Y.W. Chang; Julie Y.H. Chan; Tak Yu Lee; Samuel H.H. Chan

doi:10.1016/0304-3940(94)91098-7

Characterization and application of microdialysis probes with an active exchange length compatible with small-size brain nuclei in the rat

Mei Yung Tsou, Wen Bin Len, Alice Y.W. Chang, Julie Y.H. Chan, Tak Yu Lee, Samuel H.H. Chan

Department of Physiology

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9 Citations (Scopus)

Abstract

We communicated the construction, characterization and application of microdialysis probes with an active exchange length that is suitable for experiments involving small-size nuclei in rat brain. Using substance P (SP) as the test substance, we determined that probeds with an active exchange length of 180-200 μm exhibited an in vitro recovery of 14.2 ± 0.8% and in vivo recovery at the nucleus tractus solitarii (NTS) of 24.9 ± 1.7% for the undecapeptide, calibrated at an infusion rate of μl/min. We also demonstrated that microinfusion of SP via these probes into the NTS allowed for a correlation of changes in tissue levels of both SP (exogenous substance) and norepinephrine (endogenous substance) with alterations in baroreceptor reflex responses (physiologic phenomenon).

Original language	English
Pages (from-to)	137-140
Number of pages	4
Journal	Neuroscience Letters
Volume	175
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(94)91098-7
Publication status	Published - 1994 Jul 4

All Science Journal Classification (ASJC) codes

Neuroscience(all)

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title = "Characterization and application of microdialysis probes with an active exchange length compatible with small-size brain nuclei in the rat",

abstract = "We communicated the construction, characterization and application of microdialysis probes with an active exchange length that is suitable for experiments involving small-size nuclei in rat brain. Using substance P (SP) as the test substance, we determined that probeds with an active exchange length of 180-200 μm exhibited an in vitro recovery of 14.2 ± 0.8% and in vivo recovery at the nucleus tractus solitarii (NTS) of 24.9 ± 1.7% for the undecapeptide, calibrated at an infusion rate of μl/min. We also demonstrated that microinfusion of SP via these probes into the NTS allowed for a correlation of changes in tissue levels of both SP (exogenous substance) and norepinephrine (endogenous substance) with alterations in baroreceptor reflex responses (physiologic phenomenon).",

author = "Tsou, {Mei Yung} and Len, {Wen Bin} and Chang, {Alice Y.W.} and Chan, {Julie Y.H.} and Lee, {Tak Yu} and Chan, {Samuel H.H.}",

note = "Funding Information: Supported in part by Research Grant NSC-83-0412-B075-005 (to J.Y.H.C.) and NSC-83-0412-B010-036 (to S.H.H.C.) from the National Science Council, Taiwan, Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1994",

month = jul,

day = "4",

doi = "10.1016/0304-3940(94)91098-7",

language = "English",

volume = "175",

pages = "137--140",

journal = "Neuroscience Letters",

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Characterization and application of microdialysis probes with an active exchange length compatible with small-size brain nuclei in the rat. / Tsou, Mei Yung; Len, Wen Bin; Chang, Alice Y.W.; Chan, Julie Y.H.; Lee, Tak Yu; Chan, Samuel H.H.

In: Neuroscience Letters, Vol. 175, No. 1-2, 04.07.1994, p. 137-140.

Research output: Contribution to journal › Article › peer-review

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AU - Chan, Julie Y.H.

AU - Lee, Tak Yu

AU - Chan, Samuel H.H.

N1 - Funding Information: Supported in part by Research Grant NSC-83-0412-B075-005 (to J.Y.H.C.) and NSC-83-0412-B010-036 (to S.H.H.C.) from the National Science Council, Taiwan, Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1994/7/4

Y1 - 1994/7/4

N2 - We communicated the construction, characterization and application of microdialysis probes with an active exchange length that is suitable for experiments involving small-size nuclei in rat brain. Using substance P (SP) as the test substance, we determined that probeds with an active exchange length of 180-200 μm exhibited an in vitro recovery of 14.2 ± 0.8% and in vivo recovery at the nucleus tractus solitarii (NTS) of 24.9 ± 1.7% for the undecapeptide, calibrated at an infusion rate of μl/min. We also demonstrated that microinfusion of SP via these probes into the NTS allowed for a correlation of changes in tissue levels of both SP (exogenous substance) and norepinephrine (endogenous substance) with alterations in baroreceptor reflex responses (physiologic phenomenon).

AB - We communicated the construction, characterization and application of microdialysis probes with an active exchange length that is suitable for experiments involving small-size nuclei in rat brain. Using substance P (SP) as the test substance, we determined that probeds with an active exchange length of 180-200 μm exhibited an in vitro recovery of 14.2 ± 0.8% and in vivo recovery at the nucleus tractus solitarii (NTS) of 24.9 ± 1.7% for the undecapeptide, calibrated at an infusion rate of μl/min. We also demonstrated that microinfusion of SP via these probes into the NTS allowed for a correlation of changes in tissue levels of both SP (exogenous substance) and norepinephrine (endogenous substance) with alterations in baroreceptor reflex responses (physiologic phenomenon).

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Characterization and application of microdialysis probes with an active exchange length compatible with small-size brain nuclei in the rat

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