Abstract
We communicated the construction, characterization and application of microdialysis probes with an active exchange length that is suitable for experiments involving small-size nuclei in rat brain. Using substance P (SP) as the test substance, we determined that probeds with an active exchange length of 180-200 μm exhibited an in vitro recovery of 14.2 ± 0.8% and in vivo recovery at the nucleus tractus solitarii (NTS) of 24.9 ± 1.7% for the undecapeptide, calibrated at an infusion rate of μl/min. We also demonstrated that microinfusion of SP via these probes into the NTS allowed for a correlation of changes in tissue levels of both SP (exogenous substance) and norepinephrine (endogenous substance) with alterations in baroreceptor reflex responses (physiologic phenomenon).
Original language | English |
---|---|
Pages (from-to) | 137-140 |
Number of pages | 4 |
Journal | Neuroscience Letters |
Volume | 175 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 1994 Jul 4 |
All Science Journal Classification (ASJC) codes
- Neuroscience(all)