Characterization and evaluation of the differentiation ability of human adipose-derived stem cells growing in scaffold-free suspension culture

Yan Hsiung Wang, Jyun Yi Wu, Pei Jung Chou, Chung Hwan Chen, Chau Zen Wang, Mei Ling Ho, Je Ken Chang, Ming-Long Yeh, Chia Hsin Chen

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background aims: Human adipose-derived stem cells (hADSCs) have become a popular stem cell source because of their abundant supplies, high differentiation ability and the fact that they present few ethical concerns. Suspension culture, a type of three-dimensional culture, is a more suitable model for mimicking cell-cell and cell-extracellular matrix interactions than is two-dimensional monolayer culture. The aim of this study was to determine the effects of suspension culture on the viability and differentiation potential of hADSCs. Methods: Different densities of hADSCs were cultured in ultra-low-attachment surface plates. The morphology and mean diameter of the resultant aggregates were determined by means of microscopy. The viability of the aggregates was evaluated with the use of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt, lactate dehydrogenase and live/dead assays. To detect osteogenesis, chondrogenesis and adipogenesis in hADSCs in suspension culture, cell aggregates were stained to determine cell function, and the expression of specific markers was evaluated through the use of real-time reverse transcriptase-polymerase chain reaction. Results: The hADSCs remained viable in suspension culture and formed cell aggregates. The diameter of the majority of the aggregates was in the range of 50-200 μm, regardless of cell density. The aggregation of the hADSCs served to maintain cell survival. In addition, the results of the histomorphometric and gene expression analyses showed that the hADSCs were more efficiently induced to differentiate into osteoblasts, chondrocytes and adipocytes in suspension culture than in two-dimensional monolayer culture. Conclusions: Suspension culture can be used to maintain cell viability and contributes to the effective differentiation of hADSCs, providing an alternative cell growth strategy for application to stem cell-based regenerative medicine.

Original languageEnglish
Pages (from-to)485-495
Number of pages11
JournalCytotherapy
Volume16
Issue number4
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Suspensions
Stem Cells
Cell Survival
Cell Culture Techniques
Tetrazolium Salts
Chondrogenesis
Adipogenesis
Regenerative Medicine
Chondrocytes
Reverse Transcriptase Polymerase Chain Reaction
Osteoblasts
L-Lactate Dehydrogenase
Adipocytes
Osteogenesis
Extracellular Matrix
Real-Time Polymerase Chain Reaction
Microscopy
Cell Count
Gene Expression
Growth

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Genetics(clinical)
  • Cell Biology
  • Transplantation
  • Cancer Research

Cite this

Wang, Yan Hsiung ; Wu, Jyun Yi ; Chou, Pei Jung ; Chen, Chung Hwan ; Wang, Chau Zen ; Ho, Mei Ling ; Chang, Je Ken ; Yeh, Ming-Long ; Chen, Chia Hsin. / Characterization and evaluation of the differentiation ability of human adipose-derived stem cells growing in scaffold-free suspension culture. In: Cytotherapy. 2014 ; Vol. 16, No. 4. pp. 485-495.
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Characterization and evaluation of the differentiation ability of human adipose-derived stem cells growing in scaffold-free suspension culture. / Wang, Yan Hsiung; Wu, Jyun Yi; Chou, Pei Jung; Chen, Chung Hwan; Wang, Chau Zen; Ho, Mei Ling; Chang, Je Ken; Yeh, Ming-Long; Chen, Chia Hsin.

In: Cytotherapy, Vol. 16, No. 4, 01.01.2014, p. 485-495.

Research output: Contribution to journalArticle

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T1 - Characterization and evaluation of the differentiation ability of human adipose-derived stem cells growing in scaffold-free suspension culture

AU - Wang, Yan Hsiung

AU - Wu, Jyun Yi

AU - Chou, Pei Jung

AU - Chen, Chung Hwan

AU - Wang, Chau Zen

AU - Ho, Mei Ling

AU - Chang, Je Ken

AU - Yeh, Ming-Long

AU - Chen, Chia Hsin

PY - 2014/1/1

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