Characterization and genetic restriction of suppressor-effector cells induced by staphylococcal enterotoxin B

Dennis D. Taub, Yee-Shin Lin, Thomas J. Rogers

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The capacity of the staphylococcal enterotoxins to stimulate all T cells bearing certain (but not all) TCR has generated a great deal of interest. This stimulation appears to involve specific binding of the toxin to class II Ags and subsequent stimulation via the TCR. Previous studies from this laboratory have demonstrated that staphylococcal enterotoxin B (SEB) induces multiple T suppressor cell populations that inhibit both primary and secondary plaque-forming cell responses. Efforts to characterize these suppressor cell populations have demonstrated that the suppressor population active early in the antibody response expresses the Lyt-1-2+ cell surface phenotype, whereas depletion analysis suggests that the population active late in an ongoing response bears the Lyt-1+2+ cell-surface markers. In the present study, enrichment for this late acting effector population with the use of sequential panning with anti-Lyt mAb reveals significant suppressive activity at both the initiation and effector phases of a 5-day Mishell-Dutton coculture. Additional experiments using I-J disparate strains of mice have demonstrated a genetic restriction at the "I-J" gene locus between the cells mediating SEB-induced suppression and their target. Depletion of SEB-primed splenocytes with anti-I-J mAb suggests that both the early and late effector cells bear I-J molecules on their surface. Taken together, these results show that SEB induces suppressor cell populations with properties similar to those exhibited by Ag-specific cell circuits.

Original languageEnglish
Pages (from-to)456-462
Number of pages7
JournalJournal of Immunology
Volume144
Issue number2
Publication statusPublished - 1990 Jan 15

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Population
staphylococcal enterotoxin B
Enterotoxins
Coculture Techniques
Antibody Formation
T-Lymphocytes
Phenotype
Genes

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

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abstract = "The capacity of the staphylococcal enterotoxins to stimulate all T cells bearing certain (but not all) TCR has generated a great deal of interest. This stimulation appears to involve specific binding of the toxin to class II Ags and subsequent stimulation via the TCR. Previous studies from this laboratory have demonstrated that staphylococcal enterotoxin B (SEB) induces multiple T suppressor cell populations that inhibit both primary and secondary plaque-forming cell responses. Efforts to characterize these suppressor cell populations have demonstrated that the suppressor population active early in the antibody response expresses the Lyt-1-2+ cell surface phenotype, whereas depletion analysis suggests that the population active late in an ongoing response bears the Lyt-1+2+ cell-surface markers. In the present study, enrichment for this late acting effector population with the use of sequential panning with anti-Lyt mAb reveals significant suppressive activity at both the initiation and effector phases of a 5-day Mishell-Dutton coculture. Additional experiments using I-J disparate strains of mice have demonstrated a genetic restriction at the {"}I-J{"} gene locus between the cells mediating SEB-induced suppression and their target. Depletion of SEB-primed splenocytes with anti-I-J mAb suggests that both the early and late effector cells bear I-J molecules on their surface. Taken together, these results show that SEB induces suppressor cell populations with properties similar to those exhibited by Ag-specific cell circuits.",
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Characterization and genetic restriction of suppressor-effector cells induced by staphylococcal enterotoxin B. / Taub, Dennis D.; Lin, Yee-Shin; Rogers, Thomas J.

In: Journal of Immunology, Vol. 144, No. 2, 15.01.1990, p. 456-462.

Research output: Contribution to journalArticle

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