Characterization and genetic variations of satellite DNAs in Acrossocheilus paradoxus (Günther, 1868) (Cyprinidae) indicate population expansion

M. C. Tseng, Tzen-Yuh Chiang, J. P. Wang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A Hin dIII repetitive DNA family from Acrossocheilus paradoxus, a cyprinid fish endemic to Taiwan, was isolated and identified as a tandem arrangement of satellites in the genomic DNA. Cross-hybridization revealed similar patterns across fish genera and two families and suggested that this repetitive DNA is a conserved satellite sequence in fish. Forty-five monomeric repeat units of the repetitive DNA were cloned and sequenced, and found to be approximately 210 base pairs long and to have an average base composition of 52.8% A+T. Alignment analysis by examining 45 cloned repeat DNA strands from 22 individuals from nine different streams suggested that this repetitive DNA is highly polymorphic. The variability of sequences was mainly attributable to point mutations within the sequences. Genetic distances in all repeated DNAs ranged from 0 to 0.129 (average, 0.06). The high levels of genotype diversity and low levels of nucleotide diversity in satellites suggest population expansion of A. paradoxus.

Original languageEnglish
Pages (from-to)1138-1153
Number of pages16
JournalJournal of Fish Biology
Volume72
Issue number5
DOIs
Publication statusPublished - 2008 Apr 1

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satellite DNA
Cyprinidae
genetic variation
DNA
fish
cyprinid
point mutation
Acrossocheilus paradoxus
genetic distance
Taiwan
mutation
genomics
genotype
hybridization
nucleotides

All Science Journal Classification (ASJC) codes

  • Aquatic Science

Cite this

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abstract = "A Hin dIII repetitive DNA family from Acrossocheilus paradoxus, a cyprinid fish endemic to Taiwan, was isolated and identified as a tandem arrangement of satellites in the genomic DNA. Cross-hybridization revealed similar patterns across fish genera and two families and suggested that this repetitive DNA is a conserved satellite sequence in fish. Forty-five monomeric repeat units of the repetitive DNA were cloned and sequenced, and found to be approximately 210 base pairs long and to have an average base composition of 52.8{\%} A+T. Alignment analysis by examining 45 cloned repeat DNA strands from 22 individuals from nine different streams suggested that this repetitive DNA is highly polymorphic. The variability of sequences was mainly attributable to point mutations within the sequences. Genetic distances in all repeated DNAs ranged from 0 to 0.129 (average, 0.06). The high levels of genotype diversity and low levels of nucleotide diversity in satellites suggest population expansion of A. paradoxus.",
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Characterization and genetic variations of satellite DNAs in Acrossocheilus paradoxus (Günther, 1868) (Cyprinidae) indicate population expansion. / Tseng, M. C.; Chiang, Tzen-Yuh; Wang, J. P.

In: Journal of Fish Biology, Vol. 72, No. 5, 01.04.2008, p. 1138-1153.

Research output: Contribution to journalArticle

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