Characterization and high-level production of xylanase from an indigenous cellulolytic bacterium Acinetobacter junii F6-02 from southern Taiwan soil

Yung Chung Lo, Wei Chung Lu, Chun Yen Chen, Wen Ming Chen, Jo Shu Chang

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Hemicellulose, accounting for up to 30% of lignocellulose, could play an important role in producing cellulosic bioenergy. Xylanase is the key enzyme capable of hydrolyzing hemicellulose to form fermentable five-carbon sugars for biofuel production. A cellulolytic bacterial strain isolated from soil in southern Taiwan is able to produce xylanase and cellulase extracellularly with a high efficiency. The xylanase produced by the isolated strain (identified as Acinetobacter junii F6-02) exhibits the highest activity at 60 °C and pH 7.0. However, the best condition for producing xylanase by the F6-02 strain was 35 °C and pH 7.0 using BHM medium supplemented with CMC (5. g/L), xylan (5. g/L), and peptone (1. g/L). Fermentative production of xylanase was greatly influenced by oxygen supply, reaching the highest level at an aeration rate of 0.3. vvm. The highest cumulative xylanase production and xylanase production rate was 317. U/ml and 8.06. U/ml/h, respectively. The lyophilized cellulolytic enzymes produced from A. junii F6-02 display better enzyme activity than the original crude enzyme extract and are able to perform long-term hydrolysis stably at a temperature of 50 °C.

Original languageEnglish
Pages (from-to)77-84
Number of pages8
JournalBiochemical Engineering Journal
Volume53
Issue number1
DOIs
Publication statusPublished - 2010 Dec 15

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Characterization and high-level production of xylanase from an indigenous cellulolytic bacterium Acinetobacter junii F6-02 from southern Taiwan soil'. Together they form a unique fingerprint.

Cite this