Abstract
Our recent work showed that Chlorella vulgaris JSC-6 can accumulate over 50% carbohydrates per dry weight, which mainly contain glucose and xylose as the monosaccharides. In this study, synthetic mixed sugars simulating the hydrolyzed biomass of C. vulgaris JSC-6 (primarily containing glucose and xylose at a ratio of 5:1-6.5:1) was used as the carbon source for bio-butanol production with Clostridium acetobutylicum ATCC824. The growth and product formation kinetics based on glucose, xylose, and mixtures of the two sugars were determined using Monod-type and Michaelis-Menten models, respectively. C. acetobutylicum ATCC824 can grow faster and produce butanol more efficiently on glucose, while the performance was markedly poorer when using xylose. The butanol production kinetics were quite similar when using glucose alone or using mixed sugars (glucose to xylose ratio. =. 5:1 or 6.5:1), resulting in a maximum butanol production rate of 0.89-0.93. g/h/L. This work demonstrated the feasibility of using the microalgae-based carbohydrates as the feedstock for biobutanol production with C. acetobutylicum ATCC824.
| Original language | English |
|---|---|
| Pages (from-to) | 220-230 |
| Number of pages | 11 |
| Journal | Biochemical Engineering Journal |
| Volume | 91 |
| DOIs | |
| Publication status | Published - 2014 Oct 5 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Biomedical Engineering
- Environmental Engineering
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Characterization and kinetics of bio-butanol production with Clostridium acetobutylicum ATCC824 using mixed sugar medium simulating microalgae-based carbohydrates. / Wang, Yue; Guo, Wan Qian; Lo, Yung Chung; Chang, Jo-Shu; Ren, Nan Qi.
In: Biochemical Engineering Journal, Vol. 91, 05.10.2014, p. 220-230.Research output: Contribution to journal › Article
TY - JOUR
T1 - Characterization and kinetics of bio-butanol production with Clostridium acetobutylicum ATCC824 using mixed sugar medium simulating microalgae-based carbohydrates
AU - Wang, Yue
AU - Guo, Wan Qian
AU - Lo, Yung Chung
AU - Chang, Jo-Shu
AU - Ren, Nan Qi
PY - 2014/10/5
Y1 - 2014/10/5
N2 - Our recent work showed that Chlorella vulgaris JSC-6 can accumulate over 50% carbohydrates per dry weight, which mainly contain glucose and xylose as the monosaccharides. In this study, synthetic mixed sugars simulating the hydrolyzed biomass of C. vulgaris JSC-6 (primarily containing glucose and xylose at a ratio of 5:1-6.5:1) was used as the carbon source for bio-butanol production with Clostridium acetobutylicum ATCC824. The growth and product formation kinetics based on glucose, xylose, and mixtures of the two sugars were determined using Monod-type and Michaelis-Menten models, respectively. C. acetobutylicum ATCC824 can grow faster and produce butanol more efficiently on glucose, while the performance was markedly poorer when using xylose. The butanol production kinetics were quite similar when using glucose alone or using mixed sugars (glucose to xylose ratio. =. 5:1 or 6.5:1), resulting in a maximum butanol production rate of 0.89-0.93. g/h/L. This work demonstrated the feasibility of using the microalgae-based carbohydrates as the feedstock for biobutanol production with C. acetobutylicum ATCC824.
AB - Our recent work showed that Chlorella vulgaris JSC-6 can accumulate over 50% carbohydrates per dry weight, which mainly contain glucose and xylose as the monosaccharides. In this study, synthetic mixed sugars simulating the hydrolyzed biomass of C. vulgaris JSC-6 (primarily containing glucose and xylose at a ratio of 5:1-6.5:1) was used as the carbon source for bio-butanol production with Clostridium acetobutylicum ATCC824. The growth and product formation kinetics based on glucose, xylose, and mixtures of the two sugars were determined using Monod-type and Michaelis-Menten models, respectively. C. acetobutylicum ATCC824 can grow faster and produce butanol more efficiently on glucose, while the performance was markedly poorer when using xylose. The butanol production kinetics were quite similar when using glucose alone or using mixed sugars (glucose to xylose ratio. =. 5:1 or 6.5:1), resulting in a maximum butanol production rate of 0.89-0.93. g/h/L. This work demonstrated the feasibility of using the microalgae-based carbohydrates as the feedstock for biobutanol production with C. acetobutylicum ATCC824.
UR - http://www.scopus.com/inward/record.url?scp=84908025934&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84908025934&partnerID=8YFLogxK
U2 - 10.1016/j.bej.2014.08.007
DO - 10.1016/j.bej.2014.08.007
M3 - Article
VL - 91
SP - 220
EP - 230
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
SN - 1369-703X
ER -