Characterization and kinetics of bio-butanol production with Clostridium acetobutylicum ATCC824 using mixed sugar medium simulating microalgae-based carbohydrates

Yue Wang, Wan Qian Guo, Yung Chung Lo, Jo-Shu Chang, Nan Qi Ren

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Our recent work showed that Chlorella vulgaris JSC-6 can accumulate over 50% carbohydrates per dry weight, which mainly contain glucose and xylose as the monosaccharides. In this study, synthetic mixed sugars simulating the hydrolyzed biomass of C. vulgaris JSC-6 (primarily containing glucose and xylose at a ratio of 5:1-6.5:1) was used as the carbon source for bio-butanol production with Clostridium acetobutylicum ATCC824. The growth and product formation kinetics based on glucose, xylose, and mixtures of the two sugars were determined using Monod-type and Michaelis-Menten models, respectively. C. acetobutylicum ATCC824 can grow faster and produce butanol more efficiently on glucose, while the performance was markedly poorer when using xylose. The butanol production kinetics were quite similar when using glucose alone or using mixed sugars (glucose to xylose ratio. =. 5:1 or 6.5:1), resulting in a maximum butanol production rate of 0.89-0.93. g/h/L. This work demonstrated the feasibility of using the microalgae-based carbohydrates as the feedstock for biobutanol production with C. acetobutylicum ATCC824.

Original languageEnglish
Pages (from-to)220-230
Number of pages11
JournalBiochemical Engineering Journal
Volume91
DOIs
Publication statusPublished - 2014 Oct 5

Fingerprint

Clostridium acetobutylicum
Microalgae
Clostridium
Butanols
Carbohydrates
Xylose
Butenes
Sugars
Glucose
Chlorella vulgaris
Kinetics
Monosaccharides
Biomass
Feedstocks
Carbon
Weights and Measures
xylose-glucose
Growth

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Environmental Engineering

Cite this

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title = "Characterization and kinetics of bio-butanol production with Clostridium acetobutylicum ATCC824 using mixed sugar medium simulating microalgae-based carbohydrates",
abstract = "Our recent work showed that Chlorella vulgaris JSC-6 can accumulate over 50{\%} carbohydrates per dry weight, which mainly contain glucose and xylose as the monosaccharides. In this study, synthetic mixed sugars simulating the hydrolyzed biomass of C. vulgaris JSC-6 (primarily containing glucose and xylose at a ratio of 5:1-6.5:1) was used as the carbon source for bio-butanol production with Clostridium acetobutylicum ATCC824. The growth and product formation kinetics based on glucose, xylose, and mixtures of the two sugars were determined using Monod-type and Michaelis-Menten models, respectively. C. acetobutylicum ATCC824 can grow faster and produce butanol more efficiently on glucose, while the performance was markedly poorer when using xylose. The butanol production kinetics were quite similar when using glucose alone or using mixed sugars (glucose to xylose ratio. =. 5:1 or 6.5:1), resulting in a maximum butanol production rate of 0.89-0.93. g/h/L. This work demonstrated the feasibility of using the microalgae-based carbohydrates as the feedstock for biobutanol production with C. acetobutylicum ATCC824.",
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Characterization and kinetics of bio-butanol production with Clostridium acetobutylicum ATCC824 using mixed sugar medium simulating microalgae-based carbohydrates. / Wang, Yue; Guo, Wan Qian; Lo, Yung Chung; Chang, Jo-Shu; Ren, Nan Qi.

In: Biochemical Engineering Journal, Vol. 91, 05.10.2014, p. 220-230.

Research output: Contribution to journalArticle

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