TY - JOUR
T1 - Characterization and kinetics of bio-butanol production with Clostridium acetobutylicum ATCC824 using mixed sugar medium simulating microalgae-based carbohydrates
AU - Wang, Yue
AU - Guo, Wan Qian
AU - Lo, Yung Chung
AU - Chang, Jo Shu
AU - Ren, Nan Qi
N1 - Funding Information:
This work was supported by Open Project of State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology ( HCK201205 ). This work is also supported by National Natural Science Foundation of China (grant nos. 51008105 and 51121062 ) and Academician Workstation Construction in Guangdong Province ( 2012B090500018 ). The authors also gratefully acknowledged the support by Taiwan's Ministry of Science and Education (MOST) under grant numbers of 103-2221-E-006-190-MY3 and 103-3113-E-006-006 .
Publisher Copyright:
© 2014 Elsevier B.V.
PY - 2014/10/5
Y1 - 2014/10/5
N2 - Our recent work showed that Chlorella vulgaris JSC-6 can accumulate over 50% carbohydrates per dry weight, which mainly contain glucose and xylose as the monosaccharides. In this study, synthetic mixed sugars simulating the hydrolyzed biomass of C. vulgaris JSC-6 (primarily containing glucose and xylose at a ratio of 5:1-6.5:1) was used as the carbon source for bio-butanol production with Clostridium acetobutylicum ATCC824. The growth and product formation kinetics based on glucose, xylose, and mixtures of the two sugars were determined using Monod-type and Michaelis-Menten models, respectively. C. acetobutylicum ATCC824 can grow faster and produce butanol more efficiently on glucose, while the performance was markedly poorer when using xylose. The butanol production kinetics were quite similar when using glucose alone or using mixed sugars (glucose to xylose ratio. =. 5:1 or 6.5:1), resulting in a maximum butanol production rate of 0.89-0.93. g/h/L. This work demonstrated the feasibility of using the microalgae-based carbohydrates as the feedstock for biobutanol production with C. acetobutylicum ATCC824.
AB - Our recent work showed that Chlorella vulgaris JSC-6 can accumulate over 50% carbohydrates per dry weight, which mainly contain glucose and xylose as the monosaccharides. In this study, synthetic mixed sugars simulating the hydrolyzed biomass of C. vulgaris JSC-6 (primarily containing glucose and xylose at a ratio of 5:1-6.5:1) was used as the carbon source for bio-butanol production with Clostridium acetobutylicum ATCC824. The growth and product formation kinetics based on glucose, xylose, and mixtures of the two sugars were determined using Monod-type and Michaelis-Menten models, respectively. C. acetobutylicum ATCC824 can grow faster and produce butanol more efficiently on glucose, while the performance was markedly poorer when using xylose. The butanol production kinetics were quite similar when using glucose alone or using mixed sugars (glucose to xylose ratio. =. 5:1 or 6.5:1), resulting in a maximum butanol production rate of 0.89-0.93. g/h/L. This work demonstrated the feasibility of using the microalgae-based carbohydrates as the feedstock for biobutanol production with C. acetobutylicum ATCC824.
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U2 - 10.1016/j.bej.2014.08.007
DO - 10.1016/j.bej.2014.08.007
M3 - Article
AN - SCOPUS:84908025934
VL - 91
SP - 220
EP - 230
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
SN - 1369-703X
ER -