Characterization, extraction and purification of lutein produced by an indigenous microalga Scenedesmus obliquus CNW-N

Ming Chang Chan, Shih Hsin Ho, Duu Jong Lee, Chun Yen Chen, Chieh Chen Huang, Jo Shu Chang

Research output: Contribution to journalArticlepeer-review

105 Citations (Scopus)

Abstract

This study aimed to improve the commercial viability of microalgae-based lutein production using an isolated microalga Scenedesmus obliquus CNW-N possessing a high lutein content of over 0.25%. Effective lutein extraction protocols, appropriate storage methods, and purification procedures were developed. Disruption of microalgae cells was most efficient with a bead-beater. The conventional saponification step was modified to reduce the overall extraction time by 24. h. Diethyl ether exhibited the best lutein extraction efficiency. Storage of the lutein extract at low temperature (4 or -20. °C) with antioxidant addition (around 0.01% BHT) can maintain 90% lutein stability after 80 days. Addition of a suitable amount of the antioxidant could promote the stability of lutein extracts under the exposure of light. The protocol developed in this work allows efficient lutein extraction from S. obliquus CNW-N at a lower cost. Further purification was employed to elevate the purity of lutein and its commercial value.

Original languageEnglish
Pages (from-to)24-31
Number of pages8
JournalBiochemical Engineering Journal
Volume78
DOIs
Publication statusPublished - 2013 Sept 5

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biotechnology
  • Environmental Engineering
  • Biomedical Engineering

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