Characterization of acquired β-lactamases and their genetic support in multidrug-resistant Pseudomonas aeruginosa isolates in Taiwan: The prevalence of unusual integrons

Jing Jou Yan, Po Ren Hsueh, Jang Jih Lu, Feng Yee Chang, Wen Chien Ko, Jiunn Jong Wu

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

Objectives: The present study was conducted to investigate acquired β-lactamases and their genetic support in 26 Pseudomonas aeruginosa isolates that were resistant to nearly all antipseudomonal drugs from six medical centres in Taiwan. Methods: Acquired β-lactamases and their genetic support were determined by PCR-based strategies. Results: Four and 16 of the 26 isolates were found to produce VIM-2 and VIM-3 metallo-β-lactamases (MBLs), respectively, and 1, 1 and 2 isolates produced OXA-17, OXA-10 and PSE-1, respectively. These bla genes are all in class 1 integrons that are probably chromosomally located. The blaVIM-3-containing integron, with a deletion between int1 and the bla VIM-3 structural gene, has six gene cassettes, bla VIM-3, a probable fosfomycin resistance determinant, aacA4, aacA4, aadB and aacA4. The blaVIM-2-containing integron, without detectable 5′-conserved segment, contains four genes cassettes (aacA7-blaVIM-2-dhfr-aacA5) and is ended by tniC. The blaOXA-102-containing integron includes a catB3 cassette and a fused gene cassette, which is made up of blaOXA-17 and a novel streptomycin-spectinomycin gene, designated aadA15. The blaOXA-17-containing integron has three gene cassettes (aacA4-catB2-blaOXA-17) but the 59-base element of the bla OXA-17 cassette is interrupted by a putative transposase gene. The blaPSE-1-containing integron has three gene cassettes, aacA4, an aadA3 -related gene designated aadA3b and blaPSE-1. PFGE revealed genetic diversity among the multidrug-resistant isolates from different hospitals. Conclusions: This study demonstrated the high prevalence of VIM-type MBLs and the presence of unusual bla -encoding integrons in multidrug-resistant P. aeruginosa isolates in Taiwan. The spread of blaVIM-2-related genes by horizontal transfer might have occurred.

Original languageEnglish
Pages (from-to)530-536
Number of pages7
JournalJournal of Antimicrobial Chemotherapy
Volume58
Issue number3
DOIs
Publication statusPublished - 2006 Sep

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

Fingerprint Dive into the research topics of 'Characterization of acquired β-lactamases and their genetic support in multidrug-resistant Pseudomonas aeruginosa isolates in Taiwan: The prevalence of unusual integrons'. Together they form a unique fingerprint.

Cite this