Characterization of lipid-protein interactions using nonquenched fluorescent liposomal nanovesicles and yeast proteome microarrays

Kuan Yi Lu, Chien Sheng Chen, Johnathan Neiswinger, Heng Zhu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Studying lipid-protein interactions is central to understanding lipid signaling, a key regulatory system in cells. To better identify lipid-binding proteins, we developed a nonquenched fluorescent (NQF) liposome that is able to carry both fluorescent molecules and a lipid of interest. By combining the strength of NQF liposomes with protein microarray technology, the method presented here facilitates high-throughput screening of lipid-protein interactions. This protocol describes how to prepare NQF liposomes and apply the fabricated liposomes to yeast proteome microarrays.

Original languageEnglish
Pages (from-to)887-891
Number of pages5
JournalCold Spring Harbor Protocols
Volume2016
Issue number10
DOIs
Publication statusPublished - 2016 Oct

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All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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