Characterization of Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene

Chih Ming Chou; Chang Jen Huang; Chu Fang Lo; Guang Hsung Kou; Chung Hsiung Wang

doi:10.1006/jipa.1996.0041

Characterization of Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene

Chih Ming Chou, Chang Jen Huang, Chu Fang Lo, Guang Hsung Kou, Chung Hsiung Wang

Department of Biotechnology and Bioindustry Sciences

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

The Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene was located in EcoRI-G (6.3 kilobase pairs; kbp) and PstI-G (4.3 kbp) fragments of its genomic DNA. A portion of 1333 nucleotides (nt) containing this gene was sequenced. An open reading frame of 735 nt encoded a 245-amino-acid-long polyhedrin. A conserved TAAG motif which is associated with transcriptional start sites was identified 51 nt upstream of the translation initiation codon of PenuNPV polyhedrin gene. A putative polyadenylation signal, AATAAA, was found 116 nt downstream of the termination codon (TAA). Comparison of the amino acid sequences of PenuNPV polyhedrin with those of other NPVs showed that PenuNPV poyhedrin was most closely related to Orgyia pseudotsugata multiple NPV (OpMNPV) polyhedrin.

Original language	English
Pages (from-to)	259-266
Number of pages	8
Journal	Journal of Invertebrate Pathology
Volume	67
Issue number	3
DOIs	https://doi.org/10.1006/jipa.1996.0041
Publication status	Published - 1996 May

All Science Journal Classification (ASJC) codes

Ecology, Evolution, Behavior and Systematics

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10.1006/jipa.1996.0041

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title = "Characterization of Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene",

abstract = "The Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene was located in EcoRI-G (6.3 kilobase pairs; kbp) and PstI-G (4.3 kbp) fragments of its genomic DNA. A portion of 1333 nucleotides (nt) containing this gene was sequenced. An open reading frame of 735 nt encoded a 245-amino-acid-long polyhedrin. A conserved TAAG motif which is associated with transcriptional start sites was identified 51 nt upstream of the translation initiation codon of PenuNPV polyhedrin gene. A putative polyadenylation signal, AATAAA, was found 116 nt downstream of the termination codon (TAA). Comparison of the amino acid sequences of PenuNPV polyhedrin with those of other NPVs showed that PenuNPV poyhedrin was most closely related to Orgyia pseudotsugata multiple NPV (OpMNPV) polyhedrin.",

author = "Chou, {Chih Ming} and Huang, {Chang Jen} and Lo, {Chu Fang} and Kou, {Guang Hsung} and Wang, {Chung Hsiung}",

note = "Funding Information: This study was supported by the National Science Council under Grant No. NSC 82-0418-B-030-002-B04, Republic of China.",

year = "1996",

month = may,

doi = "10.1006/jipa.1996.0041",

language = "English",

volume = "67",

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AU - Chou, Chih Ming

AU - Huang, Chang Jen

AU - Lo, Chu Fang

AU - Kou, Guang Hsung

AU - Wang, Chung Hsiung

N1 - Funding Information: This study was supported by the National Science Council under Grant No. NSC 82-0418-B-030-002-B04, Republic of China.

PY - 1996/5

Y1 - 1996/5

N2 - The Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene was located in EcoRI-G (6.3 kilobase pairs; kbp) and PstI-G (4.3 kbp) fragments of its genomic DNA. A portion of 1333 nucleotides (nt) containing this gene was sequenced. An open reading frame of 735 nt encoded a 245-amino-acid-long polyhedrin. A conserved TAAG motif which is associated with transcriptional start sites was identified 51 nt upstream of the translation initiation codon of PenuNPV polyhedrin gene. A putative polyadenylation signal, AATAAA, was found 116 nt downstream of the termination codon (TAA). Comparison of the amino acid sequences of PenuNPV polyhedrin with those of other NPVs showed that PenuNPV poyhedrin was most closely related to Orgyia pseudotsugata multiple NPV (OpMNPV) polyhedrin.

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Characterization of Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene

Abstract

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