TY - JOUR
T1 - Characterization of serum-free ex vivo-expanded hematopoietic stem cells derived from human umbilical cord blood CD133+ cells
AU - Yao, Chao Ling
AU - Feng, Yin Hsun
AU - Lin, Xi Zhang
AU - Chu, I. Ming
AU - Hsieh, Tzu Bou
AU - Hwang, Shiaw Min
PY - 2006/2
Y1 - 2006/2
N2 - The development of ex vivo expansion of hematopoietic stem cells (HSCs) is a promising approach to restore the required bone marrow function of patients with hematological disorders. Previously, we have reported the development of an optimized serum-free and cytokines-limited defined medium using statistic methodology for umbilical cord blood-derived HSC expansion. The aim of this study was to analyze further the characteristics and functions of cells in vitro and in vivo when cultured in this defined medium. After a 7-day batch culture, the average absolute fold expansions for CD133+ cells, CD34 +CD133+ cells, CD34+CD38- cells, CB133+CB38- cells, CD34+CXCR4+ cells, CD133+CXCR4+ cells, and long-term culture-initiating cells were 21-, 20-, 723-, 618-, 160-, 384-, and 8-fold, respectively. The high enrichment of CB38- cells and CXCR4 + cells of the CD34+ subpopulation provided a very early uncommitted HSC proliferation and homing ability. Furthermore, the expanded cells showed a high level of telomerase activity to maintain their telomere length and repopulated the lethally irradiated NOD/SCID mice in vivo. These results indicated that the cytokines-limited expanded cells from CB133 + cells could substantially support simultaneous expansion of various stem/progenitor cells and engraft with the expanded cells from a low number of HSCs initially.
AB - The development of ex vivo expansion of hematopoietic stem cells (HSCs) is a promising approach to restore the required bone marrow function of patients with hematological disorders. Previously, we have reported the development of an optimized serum-free and cytokines-limited defined medium using statistic methodology for umbilical cord blood-derived HSC expansion. The aim of this study was to analyze further the characteristics and functions of cells in vitro and in vivo when cultured in this defined medium. After a 7-day batch culture, the average absolute fold expansions for CD133+ cells, CD34 +CD133+ cells, CD34+CD38- cells, CB133+CB38- cells, CD34+CXCR4+ cells, CD133+CXCR4+ cells, and long-term culture-initiating cells were 21-, 20-, 723-, 618-, 160-, 384-, and 8-fold, respectively. The high enrichment of CB38- cells and CXCR4 + cells of the CD34+ subpopulation provided a very early uncommitted HSC proliferation and homing ability. Furthermore, the expanded cells showed a high level of telomerase activity to maintain their telomere length and repopulated the lethally irradiated NOD/SCID mice in vivo. These results indicated that the cytokines-limited expanded cells from CB133 + cells could substantially support simultaneous expansion of various stem/progenitor cells and engraft with the expanded cells from a low number of HSCs initially.
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U2 - 10.1089/scd.2006.15.70
DO - 10.1089/scd.2006.15.70
M3 - Article
C2 - 16522164
AN - SCOPUS:33645417001
SN - 1547-3287
VL - 15
SP - 70
EP - 78
JO - Stem Cells and Development
JF - Stem Cells and Development
IS - 1
ER -