Characterization of the inhibitory effect of gastrodigenin and gastrodin on M-type K+ currents in pituitary cells and hippocampal neurons

Chih Sheng Yang, Ming Chi Lai, Ping Yen Liu, Yi Ching Lo, Chin Wei Huang, Sheng Nan Wu

Research output: Contribution to journalArticle

Abstract

Gastrodigenin (HBA) and gastrodin (GAS) are phenolic ingredients found in Gastrodia elata Blume (GEB), a traditional Chinese herbal medicine. These compounds have been previously used to treat cognitive dysfunction, convulsion, and dizziness. However, at present, there is no available information regarding their potential ionic effects in electrically excitable cells. In the current study, the possible effects of HBA and GAS on different ionic currents in pituitary GH3 cells and hippocampal mHippoE-14 neurons were investigated using the patch-clamp technique. The addition of HBA or GAS resulted in the differential inhibition of the M-type K+ current (IK(M)) density in a concentration-dependent manner in GH3 cells. HBA resulted in a slowing of the activation time course of IK(M), while GAS elevated it. HBA also mildly suppressed the density of erg-mediated or the delayed-rectifier K+ current in GH3 cells. Neither GAS nor HBA (10 µM) modified the voltage-gated Na+ current density, although they suppressed the L-type Ca2+ current density at the same concentration. In hippocampal mHippoE-14 neurons, HBA was effective at inhibiting IK(M) density as well as slowing the activation time course. Taken together, the present study provided the first evidence that HBA or GAS could act on cellular mechanisms, and could therefore potentially have a functional influence in various neurologic disorders.

Original languageEnglish
Article number117
JournalInternational journal of molecular sciences
Volume21
Issue number1
DOIs
Publication statusPublished - 2020 Jan 1

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neurons
Neurons
Current density
Chemical activation
Clamping devices
cells
dizziness
convulsions
activation
current density
clamps
rectifiers
Gastrodia
Electric potential
medicine
ingredients
Herbal Medicine
Dizziness
Patch-Clamp Techniques
disorders

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

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title = "Characterization of the inhibitory effect of gastrodigenin and gastrodin on M-type K+ currents in pituitary cells and hippocampal neurons",
abstract = "Gastrodigenin (HBA) and gastrodin (GAS) are phenolic ingredients found in Gastrodia elata Blume (GEB), a traditional Chinese herbal medicine. These compounds have been previously used to treat cognitive dysfunction, convulsion, and dizziness. However, at present, there is no available information regarding their potential ionic effects in electrically excitable cells. In the current study, the possible effects of HBA and GAS on different ionic currents in pituitary GH3 cells and hippocampal mHippoE-14 neurons were investigated using the patch-clamp technique. The addition of HBA or GAS resulted in the differential inhibition of the M-type K+ current (IK(M)) density in a concentration-dependent manner in GH3 cells. HBA resulted in a slowing of the activation time course of IK(M), while GAS elevated it. HBA also mildly suppressed the density of erg-mediated or the delayed-rectifier K+ current in GH3 cells. Neither GAS nor HBA (10 µM) modified the voltage-gated Na+ current density, although they suppressed the L-type Ca2+ current density at the same concentration. In hippocampal mHippoE-14 neurons, HBA was effective at inhibiting IK(M) density as well as slowing the activation time course. Taken together, the present study provided the first evidence that HBA or GAS could act on cellular mechanisms, and could therefore potentially have a functional influence in various neurologic disorders.",
author = "Yang, {Chih Sheng} and Lai, {Ming Chi} and Liu, {Ping Yen} and Lo, {Yi Ching} and Huang, {Chin Wei} and Wu, {Sheng Nan}",
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T1 - Characterization of the inhibitory effect of gastrodigenin and gastrodin on M-type K+ currents in pituitary cells and hippocampal neurons

AU - Yang, Chih Sheng

AU - Lai, Ming Chi

AU - Liu, Ping Yen

AU - Lo, Yi Ching

AU - Huang, Chin Wei

AU - Wu, Sheng Nan

PY - 2020/1/1

Y1 - 2020/1/1

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