Characterization of vasorelaxant principles from the needles of pinus morrisonicola hayata

Guan Heng Chen, Yue Chiun Li, Nan Hei Lin, Ping Chung Kuo, Jason T.C. Tzen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Pinus morrisonicola Hayata, usually called Taiwan five-leaf pine (5LP), is an endemic species in Taiwan and is traditionally used to relieve hypertension symptoms and improve cardiovascular function. In this study, the needle extract of 5LP was fractionated and analyzed by LC/MS/MS to search for possible antihypertensive candidates. In addition, bioassay-guided purification of the bioactive components was performed by Ca2+ fluorescent signal (Fluo 4-AM) assays. Two dihydrobenzofuran lignans, pinumorrisonide A (1) and icariside E4 (2), and one acylated flavonoid glycoside, kaempferol 3-O-α-(6-p-coumaroylglucosyl-β-1,4-rhamnoside) (3) were characterized from the active fractions. The structure of a new compound 1 was established on the basis of 2D NMR spectroscopic and mass spectrometric analyses, and the known compounds 2 and 3 were identified by comparison of their physical and spectroscopic data with those reported in the literature. The purified compounds 1–3 exhibited significant inhibition of Ca2+ fluorescence with IC50 values of 0.71, 0.36, and 0.20 mM, respectively. A mechanism study showed that these compounds showed vasorelaxant effects by blocking the voltage-operated Ca2+ channel (VOCC) and inhibiting Ca2+ influx to the cytoplasmic. These results suggested that 5LP and the three characterized components could be promising antihypertensive candidates for the use as VOCC blockers.

Original languageEnglish
Article number86
JournalMolecules
Volume23
Issue number1
DOIs
Publication statusPublished - 2018 Jan 1

Fingerprint

Pinus
Taiwan
Vasodilator Agents
needles
Needles
Antihypertensive Agents
hypertension
Lignans
glucosides
bioassay
Bioassay
Electric potential
electric potential
Glycosides
activity (biology)
Flavonoids
purification
Biological Assay
leaves
Inhibitory Concentration 50

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Chen, Guan Heng ; Li, Yue Chiun ; Lin, Nan Hei ; Kuo, Ping Chung ; Tzen, Jason T.C. / Characterization of vasorelaxant principles from the needles of pinus morrisonicola hayata. In: Molecules. 2018 ; Vol. 23, No. 1.
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Characterization of vasorelaxant principles from the needles of pinus morrisonicola hayata. / Chen, Guan Heng; Li, Yue Chiun; Lin, Nan Hei; Kuo, Ping Chung; Tzen, Jason T.C.

In: Molecules, Vol. 23, No. 1, 86, 01.01.2018.

Research output: Contribution to journalArticle

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