Chemical investigation of phyllanthus reticulatus by HPLC-SPE-NMR and conventional methods

Sio Hong Lam, Chen Yu Wang, Chien Kuang Chen, Shoei Sheng Lee

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)


Eight compounds, including two fiavonoid glycosides, were isolated from the butanol-soluble fraction of the methanolic extract of the leaves of Phyllanthus reticulatus by conventional methods. A polyphenol-rich fraction, obtained by Sephadex LH-20 fractionation, was also studied using an HPLC-SPE-NMR technique leading to the characterization of six compounds including three additional flavonoid glycosides. The latter approach used only 1 mg of samples, theoretically equivalent to 0.3 g of dry leaves. This study demonstrates that HPLC-SPE-NMR is very useful for thorough chemical investigation and also offers the advantages of saving time, plant materials and consumables over more conventional methods.

Original languageEnglish
Pages (from-to)251-255
Number of pages5
JournalPhytochemical Analysis
Issue number3
Publication statusPublished - 2007 May 1

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science
  • Biochemistry
  • Molecular Medicine
  • Plant Science
  • Drug Discovery
  • Complementary and alternative medicine


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