Eight compounds, including two fiavonoid glycosides, were isolated from the butanol-soluble fraction of the methanolic extract of the leaves of Phyllanthus reticulatus by conventional methods. A polyphenol-rich fraction, obtained by Sephadex LH-20 fractionation, was also studied using an HPLC-SPE-NMR technique leading to the characterization of six compounds including three additional flavonoid glycosides. The latter approach used only 1 mg of samples, theoretically equivalent to 0.3 g of dry leaves. This study demonstrates that HPLC-SPE-NMR is very useful for thorough chemical investigation and also offers the advantages of saving time, plant materials and consumables over more conventional methods.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Food Science
- Molecular Medicine
- Plant Science
- Drug Discovery
- Complementary and alternative medicine