Chemo- and enzymatic synthesis of partially and fully N-deacetylated 4-methylumbelliferyl chitobiosides: Fluorogenic substrates for chitinase

Yuji Honda, Shinji Tanimori, Mitsunori Kirihata, Satoshi Kaneko, Ken Tokuyasu, Masayuki Hashimoto, Takeshi Watanabe

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Partially and fully N-deacetylated 4-methylumberlliferyl chitobioside (1) derivatives, such as GlcN-GlcNAc-UMB (2), GlcNAc-GlcN-UMB (3), and (GlcN)2-UMB (4), were synthesized using chemo- and enzymatic procedure. Fluorescent aglycon was released from the chitobiosides 1, 2 and 3 by the action of chitinase. These UMB glycosides of heterochitobiose were versatile probes for the investigation of substrate binding chitinase from various sources. (C) 2000 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)827-829
Number of pages3
JournalBioorganic and Medicinal Chemistry Letters
Volume10
Issue number8
DOIs
Publication statusPublished - 2000 Apr

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

Fingerprint

Dive into the research topics of 'Chemo- and enzymatic synthesis of partially and fully N-deacetylated 4-methylumbelliferyl chitobiosides: Fluorogenic substrates for chitinase'. Together they form a unique fingerprint.

Cite this