Chitosan production from Paecilomyces saturatus using three monosaccharides via mixture design

Chen Hsueh Lin, Po Hsin Wang, Ten Chin Wen

Research output: Contribution to journalArticle

Abstract

In this study, we demonstrate that chitosan is produced from Paecilomyces saturatus fungi using ternary monosaccharide carbon sources liquid cultivation via mixture design strategy. Sixteen experiments were carried out to obtain regression equations of fungal dry mycelial biomass (W), chitosan ratio (R), and deacetylation degree (DD) for plotting contour lines. Contour lines reveal that the maximum W, R, and DD can be simultaneously obtained in cultivated media containing 20% glucose, 60% fructose and 20% mannitol rather than pure monosaccharide cultivation. Three additional confirmation experiments based on the maximum FuCS deacetylation degree had been performed to confirm to be 92.3% via Fourier-transform infrared spectra. Accordingly, FuCS possessed much better anti-microbial activity on E. coli than commercial chitosan (CrCS). Meanwhile, X-ray diffraction results confirmed that FuCS possessed both α and γ crystalline peaks while CrCS possessed only α crystalline peak, being collaborated with thermogravimetric analysis results. The superior FuCS was obtained by using ternary monosaccharides system in fungal culture via mixture design for the first time. This study provides a new approach to produce chitosan from fungal cultivation by using the mixture design strategy.

Original languageEnglish
Pages (from-to)307-312
Number of pages6
JournalInternational Journal of Biological Macromolecules
Volume141
DOIs
Publication statusPublished - 2019 Dec 1

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Paecilomyces
Monosaccharides
Chitosan
Crystalline materials
Fructose
Mannitol
Fourier Analysis
Fungi
X-Ray Diffraction
Biomass
Escherichia coli
Glucose
Thermogravimetric analysis
Fourier transforms
Carbon
Experiments
Infrared radiation
X ray diffraction
Liquids

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Economics and Econometrics
  • Energy(all)

Cite this

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abstract = "In this study, we demonstrate that chitosan is produced from Paecilomyces saturatus fungi using ternary monosaccharide carbon sources liquid cultivation via mixture design strategy. Sixteen experiments were carried out to obtain regression equations of fungal dry mycelial biomass (W), chitosan ratio (R), and deacetylation degree (DD) for plotting contour lines. Contour lines reveal that the maximum W, R, and DD can be simultaneously obtained in cultivated media containing 20{\%} glucose, 60{\%} fructose and 20{\%} mannitol rather than pure monosaccharide cultivation. Three additional confirmation experiments based on the maximum FuCS deacetylation degree had been performed to confirm to be 92.3{\%} via Fourier-transform infrared spectra. Accordingly, FuCS possessed much better anti-microbial activity on E. coli than commercial chitosan (CrCS). Meanwhile, X-ray diffraction results confirmed that FuCS possessed both α and γ crystalline peaks while CrCS possessed only α crystalline peak, being collaborated with thermogravimetric analysis results. The superior FuCS was obtained by using ternary monosaccharides system in fungal culture via mixture design for the first time. This study provides a new approach to produce chitosan from fungal cultivation by using the mixture design strategy.",
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Chitosan production from Paecilomyces saturatus using three monosaccharides via mixture design. / Lin, Chen Hsueh; Wang, Po Hsin; Wen, Ten Chin.

In: International Journal of Biological Macromolecules, Vol. 141, 01.12.2019, p. 307-312.

Research output: Contribution to journalArticle

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