Chloride-dependent inhibitory postsynaptic potentials recorded in amygdalar slices of rat

P. W. Gean, C. R. Hung

Research output: Contribution to journalArticle

Abstract

The physiological and pharmacological properties of inhibitory postsynaptic potentials (IPSPs) were studied in rat amygdalar slices using intracellular recording techniques. Stimulation of the ventral endopyriform nucleus evoked an excitatory postsynaptic potential (EPSP)-IPSP sequence. Depolarization of the membrane potential increased the amplitude of IPSPs, whereas hyperpolarization of the membrane potential decreased the amplitude of IPSPs. The IPSP reversed polarity (E(IPSP)) at -72 mV which is close to the equilibrium potential for chloride ions. Application of bicuculline or picrotoxin blocked the IPSP leading to the development of bursting discharges. Impalement with electrode containing 3 M KCl revealed spontaneous reversed IPSPs and caused a 10 mV depolarizing shift in E(IPSP), corresponding to an increase in intracellular Cl- concentration from 8.7 mM to 13.6 mM. It is concluded that the IPSP is mediated by the activation of GABA(A) receptors through the opening of chloride channels and that GABAergic IPSPs play an important role in controlling the excitability of amygdalar neurons.

Original languageEnglish
Pages (from-to)61-66
Number of pages6
JournalAsia Pacific Journal of Pharmacology
Volume7
Issue number1
Publication statusPublished - 1992 Jan 1

All Science Journal Classification (ASJC) codes

  • Pharmacology

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