Chromatin remodeling at the Th2 cytokine gene loci in human type 2 helper T cells

Takaaki Kaneko, Hiroyuki Hosokawa, Masakatsu Yamashita, Chrong-Reen Wang, Akihiro Hasegawa, Motoko Y. Kimura, Masayuki Kitajiama, Fumio Kimura, Masaru Miyazaki, Toshinori Nakayama

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Abstract

The differentiation of mouse naïve CD4 T cells into type 2 helper (Th2) cells is accompanied by chromatin remodeling at the nucleosomes associated with the IL-4, IL-13 and IL-5 genes. However, little is known about how chromatin remodeling of these Th2 cytokine gene loci occurs in human Th2 cells. We herein established an in vitro culture system in which both Th1 and Th2 cells are efficiently differentiated from human peripheral blood naïve CD4 T cells. This system allowed us to investigate the chromatin status at the Th2 cytokine gene loci and the IFNγ locus in human Th2 and Th1 cells, respectively. In typical individuals, the chromatin remodeling indicated by the induction of hyper-acetylation of histone H3 lysine 9 and hyper-methylation of histone H3 lysine 4 was induced at the whole Th2 cytokine gene loci in developing Th2 cells. We more precisely assessed the methylation status of histone H3 lysine 4 at the Th2 cytokine gene loci (IL-5 exon 3, IL-5 promoter, IL-5/RAD50 intergenic region, RAD50 promoter, CGRE, CNS1, IL-13 promoter, IL-4 promoter, and VA enhancer regions) and the IFNγ locus in developing Th1 and Th2 cells prepared from 20 healthy volunteers. Th2-cell specific chromatin remodeling was induced at most of the Th2 cytokine gene loci. In parallel with the induction of chromatin remodeling, GATA3 mRNA was preferentially expressed in developing Th2 cells, whereas T-bet, HLX and ROG mRNA was selectively expressed in developing Th1 cells.

Original languageEnglish
Pages (from-to)2249-2256
Number of pages8
JournalMolecular Immunology
Volume44
Issue number9
DOIs
Publication statusPublished - 2007 Mar 1

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Th2 Cells
Chromatin Assembly and Disassembly
Cytokines
Th1 Cells
Interleukin-5
Genes
Histones
Lysine
Interleukin-13
Interleukin-4
Methylation
T-Lymphocytes
Messenger RNA
Intergenic DNA
Nucleosomes
Acetylation
Helper-Inducer T-Lymphocytes
Chromatin
Exons
Healthy Volunteers

All Science Journal Classification (ASJC) codes

  • Immunology
  • Molecular Biology

Cite this

Kaneko, T., Hosokawa, H., Yamashita, M., Wang, C-R., Hasegawa, A., Kimura, M. Y., ... Nakayama, T. (2007). Chromatin remodeling at the Th2 cytokine gene loci in human type 2 helper T cells. Molecular Immunology, 44(9), 2249-2256. https://doi.org/10.1016/j.molimm.2006.11.004
Kaneko, Takaaki ; Hosokawa, Hiroyuki ; Yamashita, Masakatsu ; Wang, Chrong-Reen ; Hasegawa, Akihiro ; Kimura, Motoko Y. ; Kitajiama, Masayuki ; Kimura, Fumio ; Miyazaki, Masaru ; Nakayama, Toshinori. / Chromatin remodeling at the Th2 cytokine gene loci in human type 2 helper T cells. In: Molecular Immunology. 2007 ; Vol. 44, No. 9. pp. 2249-2256.
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Kaneko, T, Hosokawa, H, Yamashita, M, Wang, C-R, Hasegawa, A, Kimura, MY, Kitajiama, M, Kimura, F, Miyazaki, M & Nakayama, T 2007, 'Chromatin remodeling at the Th2 cytokine gene loci in human type 2 helper T cells', Molecular Immunology, vol. 44, no. 9, pp. 2249-2256. https://doi.org/10.1016/j.molimm.2006.11.004

Chromatin remodeling at the Th2 cytokine gene loci in human type 2 helper T cells. / Kaneko, Takaaki; Hosokawa, Hiroyuki; Yamashita, Masakatsu; Wang, Chrong-Reen; Hasegawa, Akihiro; Kimura, Motoko Y.; Kitajiama, Masayuki; Kimura, Fumio; Miyazaki, Masaru; Nakayama, Toshinori.

In: Molecular Immunology, Vol. 44, No. 9, 01.03.2007, p. 2249-2256.

Research output: Contribution to journalArticle

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AU - Kaneko, Takaaki

AU - Hosokawa, Hiroyuki

AU - Yamashita, Masakatsu

AU - Wang, Chrong-Reen

AU - Hasegawa, Akihiro

AU - Kimura, Motoko Y.

AU - Kitajiama, Masayuki

AU - Kimura, Fumio

AU - Miyazaki, Masaru

AU - Nakayama, Toshinori

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