Chronic and repeated Chlamydophila pneumoniae lung infection can result in increasing IL-4 gene expression and thickness of airway subepithelial basement membrane in mice

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background/Purpose: Chlamydophila pneumoniae infection has been associated with several pulmonary cardiac diseases. However, it has not been explored for its ability to activate the same immunopathologic mechanisms of asthma, namely, a predominant Th2 immune response and structural changes that are termed airway remodeling. This study evaluated immune responses in the lung and airway pathology of BALB/c mice with chronic and repeated C. pneumoniae infections. Methods: Mice were inoculated intranasally with 5 × 106 inclusion-forming units of C. pneumoniae TWAR strain, and re-inoculated at 14 and 42 days after the primary inoculation. Cytokine gene expression in bronchoalveolar lavage (BAL) cells was analyzed by RT-PCR on day 70. Airway pathology was also evaluated by morphometric measurements. Results: A significant increase of interleukin (IL)-4 mRNA was detected in BAL cells in infected mice, and a significant increase in subepithelial basement membrane thickness of the airways was also noted in infected mice as compared with control mice (8.95 ± 0.28 μm vs. 5.54±0.22 μm, p<0.0001). We further analyzed the correlation between IL-4 cytokine expression and the increased subepithelial basement membrane thickness of airways in infected mice. We found that mice with increased IL-4 mRNA expression had significant increases in the thickness of subepithelial basement membrane as compared with mice without increased IL-4 mRNA expression (9.87 ± 0.51 μm vs. 6.49 ± 0.52 μm, p < 0.0001). Conclusion: It is believed that our results demonstrated for the first time that chronic and repeated infections with C. pneumoniae increased IL-4 gene expression and thickness of airway subepithelial basement membrane in mice.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalJournal of the Formosan Medical Association
Volume108
Issue number1
DOIs
Publication statusPublished - 2009 Jan 1

Fingerprint

Chlamydophila pneumoniae
Basement Membrane
Interleukin-4
Gene Expression
Lung
Infection
Bronchoalveolar Lavage
Messenger RNA
Pathology
Cytokines
Airway Remodeling
Lung Diseases
Heart Diseases
Asthma
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

@article{88cfaab2df3445a0933bd78874fe494a,
title = "Chronic and repeated Chlamydophila pneumoniae lung infection can result in increasing IL-4 gene expression and thickness of airway subepithelial basement membrane in mice",
abstract = "Background/Purpose: Chlamydophila pneumoniae infection has been associated with several pulmonary cardiac diseases. However, it has not been explored for its ability to activate the same immunopathologic mechanisms of asthma, namely, a predominant Th2 immune response and structural changes that are termed airway remodeling. This study evaluated immune responses in the lung and airway pathology of BALB/c mice with chronic and repeated C. pneumoniae infections. Methods: Mice were inoculated intranasally with 5 × 106 inclusion-forming units of C. pneumoniae TWAR strain, and re-inoculated at 14 and 42 days after the primary inoculation. Cytokine gene expression in bronchoalveolar lavage (BAL) cells was analyzed by RT-PCR on day 70. Airway pathology was also evaluated by morphometric measurements. Results: A significant increase of interleukin (IL)-4 mRNA was detected in BAL cells in infected mice, and a significant increase in subepithelial basement membrane thickness of the airways was also noted in infected mice as compared with control mice (8.95 ± 0.28 μm vs. 5.54±0.22 μm, p<0.0001). We further analyzed the correlation between IL-4 cytokine expression and the increased subepithelial basement membrane thickness of airways in infected mice. We found that mice with increased IL-4 mRNA expression had significant increases in the thickness of subepithelial basement membrane as compared with mice without increased IL-4 mRNA expression (9.87 ± 0.51 μm vs. 6.49 ± 0.52 μm, p < 0.0001). Conclusion: It is believed that our results demonstrated for the first time that chronic and repeated infections with C. pneumoniae increased IL-4 gene expression and thickness of airway subepithelial basement membrane in mice.",
author = "Chiung-Zuei Chen and Bei-Chang Yang and Lin, {Tsun Mei} and Cheng-Hung Lee and Tzuen-Ren Hsiue",
year = "2009",
month = "1",
day = "1",
doi = "10.1016/S0929-6646(09)60031-0",
language = "English",
volume = "108",
pages = "45--52",
journal = "Journal of the Formosan Medical Association",
issn = "0929-6646",
publisher = "Excerpta Medica Asia Ltd.",
number = "1",

}

TY - JOUR

T1 - Chronic and repeated Chlamydophila pneumoniae lung infection can result in increasing IL-4 gene expression and thickness of airway subepithelial basement membrane in mice

AU - Chen, Chiung-Zuei

AU - Yang, Bei-Chang

AU - Lin, Tsun Mei

AU - Lee, Cheng-Hung

AU - Hsiue, Tzuen-Ren

PY - 2009/1/1

Y1 - 2009/1/1

N2 - Background/Purpose: Chlamydophila pneumoniae infection has been associated with several pulmonary cardiac diseases. However, it has not been explored for its ability to activate the same immunopathologic mechanisms of asthma, namely, a predominant Th2 immune response and structural changes that are termed airway remodeling. This study evaluated immune responses in the lung and airway pathology of BALB/c mice with chronic and repeated C. pneumoniae infections. Methods: Mice were inoculated intranasally with 5 × 106 inclusion-forming units of C. pneumoniae TWAR strain, and re-inoculated at 14 and 42 days after the primary inoculation. Cytokine gene expression in bronchoalveolar lavage (BAL) cells was analyzed by RT-PCR on day 70. Airway pathology was also evaluated by morphometric measurements. Results: A significant increase of interleukin (IL)-4 mRNA was detected in BAL cells in infected mice, and a significant increase in subepithelial basement membrane thickness of the airways was also noted in infected mice as compared with control mice (8.95 ± 0.28 μm vs. 5.54±0.22 μm, p<0.0001). We further analyzed the correlation between IL-4 cytokine expression and the increased subepithelial basement membrane thickness of airways in infected mice. We found that mice with increased IL-4 mRNA expression had significant increases in the thickness of subepithelial basement membrane as compared with mice without increased IL-4 mRNA expression (9.87 ± 0.51 μm vs. 6.49 ± 0.52 μm, p < 0.0001). Conclusion: It is believed that our results demonstrated for the first time that chronic and repeated infections with C. pneumoniae increased IL-4 gene expression and thickness of airway subepithelial basement membrane in mice.

AB - Background/Purpose: Chlamydophila pneumoniae infection has been associated with several pulmonary cardiac diseases. However, it has not been explored for its ability to activate the same immunopathologic mechanisms of asthma, namely, a predominant Th2 immune response and structural changes that are termed airway remodeling. This study evaluated immune responses in the lung and airway pathology of BALB/c mice with chronic and repeated C. pneumoniae infections. Methods: Mice were inoculated intranasally with 5 × 106 inclusion-forming units of C. pneumoniae TWAR strain, and re-inoculated at 14 and 42 days after the primary inoculation. Cytokine gene expression in bronchoalveolar lavage (BAL) cells was analyzed by RT-PCR on day 70. Airway pathology was also evaluated by morphometric measurements. Results: A significant increase of interleukin (IL)-4 mRNA was detected in BAL cells in infected mice, and a significant increase in subepithelial basement membrane thickness of the airways was also noted in infected mice as compared with control mice (8.95 ± 0.28 μm vs. 5.54±0.22 μm, p<0.0001). We further analyzed the correlation between IL-4 cytokine expression and the increased subepithelial basement membrane thickness of airways in infected mice. We found that mice with increased IL-4 mRNA expression had significant increases in the thickness of subepithelial basement membrane as compared with mice without increased IL-4 mRNA expression (9.87 ± 0.51 μm vs. 6.49 ± 0.52 μm, p < 0.0001). Conclusion: It is believed that our results demonstrated for the first time that chronic and repeated infections with C. pneumoniae increased IL-4 gene expression and thickness of airway subepithelial basement membrane in mice.

UR - http://www.scopus.com/inward/record.url?scp=60149109947&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60149109947&partnerID=8YFLogxK

U2 - 10.1016/S0929-6646(09)60031-0

DO - 10.1016/S0929-6646(09)60031-0

M3 - Article

VL - 108

SP - 45

EP - 52

JO - Journal of the Formosan Medical Association

JF - Journal of the Formosan Medical Association

SN - 0929-6646

IS - 1

ER -